miR-30a inhibits endothelin A receptor and chemoresistance in ovarian carcinoma
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Rosanna Sestito1,*, Roberta Cianfrocca1,*, Laura Rosanò1, Piera Tocci1, Elisa Semprucci1, Valeriana Di Castro1, Valentina Caprara1, Gabriella Ferrandina2, Andrea Sacconi3, Giovanni Blandino3, Anna Bagnato1
1Translational Research Functional Departmental Area, Regina Elena National Cancer Institute, Rome, Italy
2Gynecologic Oncology Unit, Catholic University of Rome, Rome, Italy
3Translational Oncogenomic Unit, Regina Elena National Cancer Institute, Rome, Italy
*These authors have contributed equally to this work
Anna Bagnato, e-mail: [email protected]
Keywords: ovarian carcinoma, endothelin A receptor, miR-30a, chemoresistance, endothelin-1
Received: September 01, 2015 Accepted: November 24, 2015 Published: December 10, 2015
Drug resistance remains the major clinical barrier to successful treatment in epithelial ovarian carcinoma (EOC) patients, and the evidence of microRNA involvement in drug resistance has been recently emerging. Endothelin-1 (ET-1)/ETA receptor (ETAR) axis is aberrantly activated in chemoresistant EOC cells and elicits pleiotropic effects promoting epithelial-to-mesenchymal transition (EMT) and the acquisition of chemoresistance. However, the relationship between ETAR and miRNA is still unknown. Hence, in this study we evaluated whether dysregulation of miRNA might enhance ETAR expression in sensitive and resistant EOC cells. Based on bioinformatic tools, we selected putative miRNA able to recognize the 3′UTR of ETAR. An inverse correlation was observed between the expression levels of miR-30a and ETAR in both EOC cell lines and tumor samples. miR-30a was found to specifically bind to the 3′UTR of ETAR mRNA, indicating that ETAR is a direct target of miR-30a. Overexpression of miR-30a decreased Akt and mitogen activated protein kinase signaling pathway activation, cell proliferation, invasion, plasticity, EMT marker levels, and vascular endothelial growth factor release. Interestingly, ectopic expression of miR-30a re-sensitized platinum-resistant EOC cells to cisplatinum-induced apoptosis. Consistently, resistant EOC xenografts overexpressing miR-30a resulted in significantly less tumor growth than controls. Together our study provides a new perspective on the regulatory mechanism of ETAR gene. Interestingly, our findings highlight that blockade of ETAR regulatory axis is the mechanism underlying the tumor suppressor function of miR-30a in chemoresistant EOC cells.
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