Oncotarget

Research Papers:

The IgH 3′ regulatory region governs μ chain transcription in mature B lymphocytes and the B cell fate

Alexis Saintamand _, Pauline Rouaud, Armand Garot, Faten Saad, Claire Carrion, Christelle Oblet, Michel Cogné, Eric Pinaud and Yves Denizot

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Oncotarget. 2015; 6:4845-4852. https://doi.org/10.18632/oncotarget.3010

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Abstract

Alexis Saintamand1, Pauline Rouaud1, Armand Garot1, Faten Saad1, Claire Carrion1, Christelle Oblet1, Michel Cogné1,2,3, Eric Pinaud1, Yves Denizot1

1CNRS, CRIBL, UMR 7276, Limoges, France

2Université de Limoges, CRIBL, UMR 7276, Limoges, France

3Institut Universitaire de France, Paris, France

Correspondence to:

Yves Denizot, e-mail: yves.denizot@unilim.fr

Keywords: BCR, B cell fate, IgH 3’ regulatory enhancers, knock-out mice

Received: December 17, 2014     Accepted: December 21, 2014     Published: March 03, 2015

ABSTRACT

We report that the IgH 3’ regulatory region (3’RR) has no role on μ chain transcription and pre-BCR expression in B cell progenitors. In contrast, analysis of heterozygous IgH aΔ3’RR/bwt mice indicated that the 3’RR controls μ chain transcripts in mature splenocytes and impacts membrane IgM density without obvious effect on BCR signals (colocalisation with lipid rafts and phosphorylation of Erk and Akt after BCR crosslinking). Deletion of the 3’RR modulates the B cell fate to less marginal zone B cells. In conclusion, the 3’RR is dispensable for pre-BCR expression and necessary for optimal commitments toward the marginal zone B cell fate. These results reinforce the concept of a dual regulation of the IgH locus transcription and accessibility by 5’ elements at immature B cell stages, and by the 3’RR as early as the resting mature B cell stage and then along further activation and differentiation.


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