GFOD1 and peejar are promising markers for clear-cell renal cell carcinoma disease progression
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Xiao-rong Wu1,*, Yong-hui Chen1,*, Wei Chen1, Wen Kong1, Ji-wei Huang1, Jin Zhang1, Wei Xue1, Dong-ming Liu1, Yi-ran Huang1
1Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China
*These authors contributed equally to this work
Yi-ran Huang, email: [email protected]
Dong-ming Liu, email: [email protected]
Keywords: gene expression profiling, long non-coding RNA, clear cell renal cell carcinoma
Received: January 12, 2016 Accepted: April 26, 2016 Published: May 15, 2016
Clear cell renal cell carcinoma (ccRCC) is a common genitourinary malignancy. The molecular pathogenesis of ccRCC remains unclear and biomarkers for daily practice were still limited. We performed an integrative analysis of two public ccRCC microarray datasets, E-GEOD-22541 and E-MTAB-1050, The candidate differential expression genes (DEG) were then confirmed in the E-GEOD-53757 dataset. In addition, an independent cohort of 50 ccRCC and 36 non-tumor kidney tissues were analyzed to examine the selected DGEs by qRT-PCR. We identified and validated two DEGs, namely GFOD1 and peejar, which were significantly up-regulated in ccRCC compared with normal renal tissues (p < 0.001). Moreover, the expression of these two genes are related to histological grade and stage and decrease of their expression correlated with disease progression (p < 0.05). Furthermore, we found the expression of peejar was positively correlated with the expression of GFOD1 in ccRCC tissue, with Pearson correlation coefficiency reaching 0.939 (p < 0.001). GFOD1 and peejar were novel genes correlated with ccRCC disease progression and patients' poor prognosis.
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