TAB3 O-GlcNAcylation promotes metastasis of triple negative breast cancer
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Tao Tao1,2, Zhixian He3, Zhiming Shao1, Haojie Lu1,2,4
1Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P.R. China
2Department of Chemistry, Fudan University, Shanghai 200433, P.R. China
3Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001, P.R. China
4Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University, Shanghai 200032, P.R. China
Haojie Lu, e-mail: [email protected]
Keywords: triple negative breast cancer, metastasis, TAB3, O-glcNAcylation
Received: October 08, 2015 Accepted: January 29, 2016 Published: March 18, 2016
O-GlcNAcylation is a post-translational modification that regulates a broad range of nuclear and cytoplasmic proteins and is emerging as a key regulator of various biological processes. Although previous studies have shown that increased levels of global O-GlcNAcylation and O-GlcNActransferase are linked to the incidence of metastasis in triple negative breast cancer (TNBC) patients, the molecular basis behind this is not fully understood. In this study, we have determined that the TAK1 binding protein 3 (TAB3) was O-GlcNAcylated at Ser408 by OGT in the TNBC, which was required for its Thr404 phosphorylation, TAK1 activation and downstream nuclear factor kappa B (NF-κB) activation in TNBC. O-GlcNAcylation of TAB3 was induced by p38 MAPK and it in turn enhances the TAK1 mediated p38MAPK activation, which forms the positive feedback loop in TAB3mediated NF-κB activation. In TNBC, TAB3O-GlcNAcylationmediated cell migration and invasion by activating its downstream NF-κB. The expression of TAB3 O-GlcNAcylation increased in TNBC patients, and it was significantly correlated with poor prognoses of the patients. Our study provides insights into the mechanism of TAB3 regulating activity and suggests its important implications in TNBC metastasis.
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