Mitochondrial ribosomal protein S18-2 is highly expressed in endometrial cancers along with free E2F1
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Miriam Mints1,*, Muhammad Mushtaq2,*, Natalia Iurchenko3, Larysa Kovalevska3, Maria C Stip2, Daria Budnikova3, Sonia Andersson1, Ludmila Polischuk3, Lubov Buchynska3, Elena Kashuba2,3
1Department of Women's and Children's Health (KBH), Karolinska Institutet, Stockholm, 17176, Sweden
2Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, 17177, Sweden
3R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, 03022, Ukraine
*The authors have equally contributed to this work
Elena Kashuba, e-mail: [email protected]
Keywords: endometrial cancer, mitochondrial ribosomal protein MRPS18-2, cell transformation, E2F1, prognostic markers
Received: January 13, 2016 Accepted: February 21, 2016 Published: March 3, 2016
Endometrial cancer (EC) is one of the most frequent causes of cancer death among women in developed countries. Histopathological diagnosis and imaging techniques for EC are limited, thus new prognostic markers are needed to offer patients the best treatment and follow-up.
In the present paper we showed that the level of mitochondrial ribosomal protein MRPS18-2 (S18-2) increased in EC compared with the normal endometrium and hyperplasia, based on a study of 42 patient biopsies. Importantly, high expression of free E2F1 in EC correlates well with high S18-2 expression. The EC cell line HEC-1-A, which overexpresses S18-2 constitutively, showed an increased proliferation capacity in vitro and in vivo (in SCID mice). Moreover, pan-keratin, beta-catenin and E-cadherin signals are diminished in these cells, compared to the parental HEC-1-A line, in contrast to vimentin signal that is increased. This may be associated with epithelial-mesenchymal cell transition (EMT).
We conclude that high expression of S18-2 and free E2F1, and low pan-keratin, beta-catenin, and E-cadherin signals might be a good set of prognostic markers for EC.
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