Research Papers:
Comparative effectiveness of light emitting diodes LEDs and Lasers in near infrared photoimmunotherapy
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Kazuhide Sato1, Rira Watanabe1, Hirofumi Hanaoka1, Takahito Nakajima1, Peter L. Choyke1, Hisataka Kobayashi1
1Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
Correspondence to:
Hisataka Kobayashi, e-mail: [email protected]
Keywords: near infrared photoimmunotherapy, light emitting diode (LED), light amplification by stimulated emission of radiation (Laser), epidermal growth factor receptor, super-enhanced permeability and retention (SUPR) effect
Received: December 03, 2015 Accepted: January 29, 2016 Published: February 13, 2016
ABSTRACT
Near infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that combines the specificity of antibodies for targeting tumors with the toxicity induced by photosensitizers after exposure to near infrared (NIR) light. Herein we compare two NIR-light sources; light emitting diodes (LEDs) and Lasers, for their effectiveness in NIR-PIT.
A photosensitizer, IRDye-700DX, conjugated to panitumumab (pan-IR700), was incubated with EGFR-expressing A431 and MDA-MB-468-luc cells. NIR-light was provided by LEDs or Lasers at the same light dose. Laser-light produced more cytotoxicity and greater reductions in IR700-fluorescence intensity than LED-light. Laser-light also produced more cytotoxicity in vivo in both cell lines. Assessment of super-enhanced permeability and retention (SUPR) effects were stronger with Laser than LED.
These results suggest that Laser-light produced significantly more cytotoxic effects compared to LEDs. Although LED is less expensive, Laser-light produces superior results in NIR-PIT.