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Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development

Chengcao Sun, Chuanfeng Huang, Shujun Li, Cuili Yang, Yongyong Xi, Liang Wang, Feng Zhang, Yunfeng Fu and Dejia Li _

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Oncotarget. 2016; 7:8341-8359. https://doi.org/10.18632/oncotarget.7071

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Abstract

Chengcao Sun1,*, Chuanfeng Huang1,2,*, Shujun Li1,3, Cuili Yang1, Yongyong Xi1, Liang Wang1, Feng Zhang1, Yunfeng Fu4, Dejia Li1

1Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China

2Department of Pharmacology, Basic Medical School, Nanyang Medical College, 473003 Nanyang, P.R.China

3Wuhan Hospital for the Prevention and Treatment of Occupational Diseases, 430071 Wuhan, P.R.China

4The Third Xiang-Ya Hospital, Central South University, 410013 Changsha, P.R.China

*These authors contributed equally to this work

Correspondence to:

Dejia Li, e-mail: [email protected]

Yunfeng Fu, e-mail: [email protected]

Keywords: Hsa-miRNA-326 (miR-326), cyclin D1, non-small cell lung cancer (NSCLC), proliferation, apoptosis

Received: August 18, 2015     Accepted: January 13, 2016     Published: January 29, 2016

ABSTRACT

Hsa-miRNA-326 (miR-326) has recently been discovered having anticancer efficacy in different organs. However, the role of miR-326 on non-small cell lung cancer (NSCLC) is still ambiguous. In this study, we investigated the role of miR-326 on the development of NSCLC. The results indicated that miR-326 was significantly down-regulated in primary tumor tissues and very low levels were found in NSCLC cell lines. Ectopic expression of miR-326 in NSCLC cell lines significantly suppressed cell growth as evidenced by cell viability assay, colony formation assay and BrdU staining, through inhibition of cyclin D1, cyclin D2, CDK4 and up-regulation of p57(Kip2) and p21(Waf1/Cip1). In addition, miR-326 induced apoptosis, as indicated by concomitantly with up-regulation of key apoptosis protein cleaved caspase-3, and down-regulation of anti-apoptosis protein Bcl2. Moreover, miR-326 inhibited cellular migration and invasiveness through inhibition of matrix metalloproteinases (MMP)-7 and MMP-9. Further, oncogene CCND1 was revealed to be a putative target of miR-326, which was inversely correlated with miR-326 expression in NSCLC. Taken together, our results demonstrated that miR-326 played a pivotal role on NSCLC through inhibiting cell proliferation, migration, invasion, and promoting apoptosis by targeting oncogenic CCND1.


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