Oncotarget

Research Papers:

SLC7A5 act as a potential leukemic transformation target gene in myelodysplastic syndrome

Yan Ma, Jing Song, Bobin Chen, Xiaoping Xu _ and Guowei Lin

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Oncotarget. 2016; 7:6566-6575. https://doi.org/10.18632/oncotarget.6512

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Abstract

Yan Ma1, Jing Song1, Bobin Chen1, Xiaoping Xu1, Guowei Lin1

1Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China

Correspondence to:

Xiaoping Xu, e-mail: xpxu1111@163.com

Keywords: myelodysplastic syndrome, miRNA, SLC7A5 gene, SKM-1 cell line, siRNA

Received: July 22, 2015     Accepted: November 21, 2015     Published: December 09, 2015

ABSTRACT

Objective: Myelodysplastic syndromes (MDS) are a heterogenous group of clonal hematopoietic stem cell disorders characterized by increased risk of leukemic transformation. This study identifies microRNAs(miRNA) and miRNA targets that might represent leukemic transformation markers for MDS.

Methods: Based on our previously established nested case-control study cohort of MDS patients, we chose paired patients to undergo Angilent 8 × 15K human miRNA microarrays. Target prediction analysis was administrated using targetscan 5.1 software. We further investigated the function of target gene in MDS cell line using siRNA method, including cell proliferation, cell apoptosis, cell cycle and electron microscope.

Results: Finally we screened a subset of 7 miRNAs to be significantly differentially expressed between the case (at the end of follow up with leukemic transformation) and control group (at the end of follow up without leukemic transformation). Target prediction analysis revealed SLC7A5 was the common target gene of these 7 miRNAs. Further study on the function of SLC7A5 gene in SKM-1 cell line showed that downregulation of SLC7A5 inhibited SKM-1 cells proliferation, increased apoptosis and caused cell cycle arrest in the G0/G1 stage.

Conclusion: Our data indicate that SLC7A5 gene may act as a potential leukemic transformation target gene in MDS.


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