Research Papers:

High throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery

Dan Wang, Kebang Hu, Ningning Gao, Hao Zhang, Yanlin Jiang, Caigang Liu _, Shouyu Wang and Zuowei Zhao

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Oncotarget. 2015; 6:29296-29310. https://doi.org/10.18632/oncotarget.4828

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Dan Wang1,*, Kebang Hu2,*, Ningning Gao3, Hao Zhang1, Yanlin Jiang1, Caigang Liu1, Shouyu Wang4, Zuowei Zhao1

1Breast Disease and Reconstruction Center, Breast Cancer Key Lab of Dalian, The Second Hospital of Dalian Medical University, Dalian, China 116023

2Department of Urology, First Hospital of Jilin University, Changchun, China 130021

3Ultrasonic Diagnosis Department, The First Hospital of China Medical University, Shenyang, China 110001

4Department of Surgery, the first Hospital of Dalian Medical University, Dalian, China 114000

*These authors have contributed equally to this work

Correspondence to:

Caigang Liu, e-mail: [email protected]

Shouyu Wang, e-mail: [email protected]

Zuowei Zhao, e-mail: [email protected]

Keywords: breast cancer, wound fluid, proliferation

Received: March 30, 2015     Accepted: July 29, 2015     Published: August 10, 2015


Objective: To clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines.

Methods: The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quantitative profiles of WF were analyzed using Bio-Plex Pro kits.

Results: The proliferation and migration of WF-treated breast cancer cells were significantly higher than that of untreated cells. Fifteen cytokines, 29 chemokines and 9 matrix metalloproteinases (MMPs) were assessed in WF. The concentrations of these factors were influenced by post-surgery days, neoadjuvant chemotherapy (NAC), TNM stage, pathological type and molecular subtype. The WF harvested from patients underwent NAC showed significant higher profiles of interleukin-1β (IL-1β), IL-4, IL-6, IL-17F, IL-21, IL-23, IL-25, IL-31, Interferon γ (IFNγ), CD40 ligand (CD40L), tumor necrosis factor α (TNFα), CXCL1, CXCL2, CXCL5, CCL3, CCL7 and CCL20.

Conclusions: Surgery-induced WF promotes the proliferation and migration of breast cancer cells. The composition of WF is influenced by various clinical features and provides potential therapeutic targets to control local recurrence and tumor progression.

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