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Down-regulation of c-Met and Bcl2 by microRNA-206, activates apoptosis, and inhibits tumor cell proliferation, migration and colony formation

Chengcao Sun, Shujun Li, Cuili Yang, Ruilin Xue, Yongyong Xi, Liang Wang, Suqing Wang, Qiqiang He, Jie Huang, Songping Xie, Wenyang Jiang and Dejia Li _

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Oncotarget. 2015; 6:25533-25574. https://doi.org/10.18632/oncotarget.4575

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Abstract

Chengcao Sun1,2,*, Zhidong Liu3,*, Shujun Li1,4,*, Cuili Yang1, Ruilin Xue1, Yongyong Xi1, Liang Wang1, Suqing Wang1, Qiqiang He1, Jie Huang5, Songping Xie5, Wenyang Jiang5, Dejia Li1

1Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan 430071, P. R. China

2Institute of Global Health, Wuhan University, Wuhan 430071, P. R. China

3Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing 101149, P.R. China

4Wuhan Hospital for the Prevention and Treatment of Occupational Diseases, Wuhan 430071, P. R. China

5Department of Thoracic Surgery, People's Hospital of Wuhan University, Wuhan 430000, P. R. China

*These authors have contributed equally to this work

Correspondence to:

Dejia Li, e-mail: [email protected]

Keywords: hsa-miRNA-206(miR-206), c-Met, Bcl2, non-small cell lung cancer (NSCLS), proliferation

Received: April 08, 2015     Accepted: July 13, 2015     Published: July 25, 2015

ABSTRACT

Hsa-miRNA-206 (miR-206), highly expressed in skeletal muscle, has recently been discovered to have anticancer properties in different tissues. However, the role of miR-206 on lung cancer is still ambiguous. In this study, we investigated the role of miR-206 on the development of lung cancer. The results indicated that miR-206 expression was suppressed in lung cancer tissues and very low levels were found in non-small cell lung cancer (NSCLS) cell liness. Transient transfection of miR-206 into cultured A549 and SK-MES-1 cells led to significant decrease in cell growth, migration, invasion and colony formation, and promoted cell apoptosis. Using bioinformatics, we identified putative miR-206 binding sites within the 3′-untranslated region (3′-UTR) of the human c-Met and Bcl2 mRNA. The expression of c-Met and Bcl2 proteins were shown to be down-regulated after treated with miR-206 by subsequent Western blot and qRT-PCR analysis. Conversely, up-regulation of c-Met and Bcl2 were confirmed in tissue samples of human lung cancer, with its level inversely correlated with miR-206 expression. In addition, miR-206 also decreased the gene expression of MMP-9, CCND1 and CCND2 while increased the gene expression of p57 (Kip2) in A549 and SK-MES-1 cells. Taken together, our results demonstrated that miR-206 suppressed c-Met and Bcl2 expression in NSCLS and could function as a potent tumor suppressor in c-Met/Bcl2-over expressing tumors. Inhibition of miR-206 function could contribute to aberrant cell proliferation, migration, invasion and apoptosis, leading to NSCLS development.


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