Androgen receptor non-nuclear regulation of prostate cancer cell invasion mediated by Src and matriptase
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Jelani C. Zarif1,2, Laura E. Lamb3, Veronique V. Schulz1, Eric A. Nollet1,4, Cindy K. Miranti1
1Laboratory of Integrin Signaling and Tumorigenesis, Van Andel Research Institute, Grand Rapids, MI 49503, USA
2Cell and Molecular Biology Program, Michigan State University, East Lansing, MI 48824, USA
3Department of Urology, Beaumont Health System - Research Institute, Royal Oak, MI 48073, USA
4Van Andel Institute Graduate School, Grand Rapids, MI 49503, USA
Cindy K. Miranti, e-mail: firstname.lastname@example.org
Keywords: prostate cancer, nongenomic AR signaling, Src, metastasis, castration-resistant
Received: July 09, 2014 Accepted: January 08, 2015 Published: January 29, 2015
Castration-resistant prostate cancers still depend on nuclear androgen receptor (AR) function despite their lack of dependence on exogenous androgen. Second generation anti-androgen therapies are more efficient at blocking nuclear AR; however resistant tumors still develop. Recent studies indicate Src is highly active in these resistant tumors. By manipulating AR activity in several different prostate cancer cell lines through RNAi, drug treatment, and the use of a nuclear-deficient AR mutant, we demonstrate that androgen acting on cytoplasmic AR rapidly stimulates Src tyrosine kinase via a non-genomic mechanism. Cytoplasmic AR, acting through Src enhances laminin integrin-dependent invasion. Active Matriptase, which cleaves laminin, is elevated within minutes after androgen stimulation, and is subsequently shed into the medium. Matriptase activation and shedding induced by cytoplasmic AR is dependent on Src. Concomitantly, CDCP1/gp140, a Matriptase and Src substrate that controls integrin-based migration, is activated. However, only inhibition of Matriptase, but not CDCP1, suppresses the AR/Src-dependent increase in invasion. Matriptase, present in conditioned medium from AR-stimulated cells, is sufficient to enhance invasion in the absence of androgen. Thus, invasion is stimulated by a rapid but sustained increase in Src activity, mediated non-genomically by cytoplasmic AR, leading to rapid activation and shedding of the laminin protease Matriptase.
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