Oncotarget

Research Papers:

Collaborating genomic, transcriptomic and microbiomic alterations lead to canine extreme intestinal polyposis

Jin Wang, Tianfang Wang, Micah A. Bishop, John F. Edwards, Hang Yin, Stephen Dalton, Laura K. Bryan and Shaying Zhao _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2018; 9:29162-29179. https://doi.org/10.18632/oncotarget.25646

Metrics: PDF 827 views  |   HTML 1463 views  |   ?  


Abstract

Jin Wang1, Tianfang Wang1, Micah A. Bishop3, John F. Edwards2, Hang Yin1, Stephen Dalton1, Laura K. Bryan2 and Shaying Zhao1

1Department of Biochemistry and Molecular Biology, Institute of Bioinformatics, University of Georgia, Athens, GA 30602, USA

2Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA

3Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA

Correspondence to:

Shaying Zhao, email: szhao@uga.edu

Laura K. Bryan, email: lbryan@cvm.tamu.edu

Keywords: canine extreme intestinal polyposis; germline and somatic mutation; MYC network activation; microbiome; B. uniformis and redox

Received: January 22, 2018     Accepted: June 01, 2018     Published: June 26, 2018

ABSTRACT

Extreme intestinal polyposis in pet dogs has not yet been reported in literature. We identified a dog patient who developed numerous intestinal polyps, with the severity resembling human classic familial adenomatous polyposis (FAP), except the jejunum-ileum junction being the most polyp-dense. We investigated this dog, in comparison with 22 other dogs with spontaneous intestinal tumors but no severe polyposis, and with numerous published human cancers. We found, not APC mutation, but three other alteration pathways as likely reasons of this canine extreme polyposis. First, somatic truncation mutation W411X of FBXW7, a component of an E3 ubiquitin ligase, over-activates MYC and cell cycle-promoting network, accelerating crypt cell proliferation. Second, genes of protein trafficking and localization are downregulated, likely associated with germline mutation G406D of STAMBPL1, a K63-deubiquitinase, and MYC network activation. This inhibits epithelial apical-basolateral polarity establishment, preventing crypt cell differentiation. Third, Bacteroides uniformis, a commensal gut anaerobe, thrives and expresses abundantly thioredoxin and nitroreductase. These bacterial products could reduce oxidative stress linked to host germline mutation R51X of CYB5RL, a cytochrome b5 reductase homologue, decreasing cell death. Our work emphasizes the close collaboration of alterations across the genome, transcriptome and microbiome in promoting tumorigenesis.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 25646