Research Papers: Immunology:

Genome-wide comparison of the protein-coding repertoire reveals fast evolution of immune-related genes in cephalochordates and Osteichthyes superclass

Qi-Lin Zhang, Bin Xu, Xiu-Qiang Wang, Ming-Long Yuan and Jun-Yuan Chen _

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2018; 9:83-95. https://doi.org/10.18632/oncotarget.22749

Metrics: PDF 1599 views  |   HTML 2559 views  |   ?  


Qi-Lin Zhang1,2, Bin Xu1, Xiu-Qiang Wang1,2, Ming-Long Yuan3 and Jun-Yuan Chen1,2

1 LPS of Nanjing Institute of Geology and Palaeontology, CAS, Nanjing, China

2 State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, China

3 State Key Laboratory of Grassland Agro-Ecosystems,College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China

Correspondence to:

Jun-Yuan Chen, email:

Ming-Long Yuan, email:

Qi-Lin Zhang, email:

Keywords: cephalochordates; Osteichthyes superclass; immune-related genes; fast evolution; protein-coding genes; Immunology

Received: May 24, 2017 Accepted: November 11, 2017 Published: November 28, 2017


Amphioxus is used to investigate the origin and evolution of vertebrates. To better understand the characteristics of genome evolution from cephalochordates to Osteichthyes, we conducted a genome-wide pairwise comparison of protein-coding genes within amphioxus (a comparable group) and parallel analyses within Osteichthyes (two comparable groups). A batch of fast-evolving genes in each comparable group was identified. Of these genes, the most fast-evolving genes (top 20) were scrutinized, most of which were involved in immune system. An analysis of the fast-evolving genes showed that they were enriched into gene ontology (GO) terms and pathways primarily involved in immune-related functions. Similarly, this phenomenon was detected within Osteichthyes, and more well-known and abundant GO terms and pathways involving innate immunity were found in Osteichthyes than in cephalochordates. Next, we measured the expression responses of four genes belonging to metabolism or energy production-related pathways to lipopolysaccharide challenge in the muscle, intestine or skin of B. belcheri; three of these genes (HMGCL, CYBS and MDH2) showed innate immune responses. Additionally, some genes involved in adaptive immunity showed fast evolution in Osteichthyes, such as those involving “intestinal immune network for IgA production” or “T-cell receptor signaling pathway”. In this study, the fast evolution of immune-related genes in amphioxus and Osteichthyes was determined, providing insights into the evolution of immune-related genes in chordates.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 22749