Oncotarget

Research Papers: Immunology:

Characterization and anti-inflammation role of swine IFITM3 gene

He-Ping Li _, Pei-Ge Chen, Fu-Tao Liu, He-Shui Zhu, Xian-Qin Jiao, Kai Zhong, Yu-Jie Guo, Guang-Ming Zha, Li-Qiang Han, Wei-Fei Lu, Yue-Ying Wang and Guo-Yu Yang

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Oncotarget. 2017; 8:73579-73589. https://doi.org/10.18632/oncotarget.20568

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Abstract

He-Ping Li1,*, Pei-Ge Chen1,*, Fu-Tao Liu1,*, He-Shui Zhu1, Xian-Qin Jiao1, Kai Zhong1, Yu-Jie Guo1, Guang-Ming Zha1, Li-Qiang Han1, Wei-Fei Lu1, Yue-Ying Wang1, Guo-Yu Yang1

1 Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China

* These authors have contributed equally to this study

Correspondence to:

Yue-Ying Wang, email:

Guo-Yu Yang, email:

Keywords: IFITM3, characterization, anti-inflammation, TLR4 signaling pathway, lipopolysaccharide, Immunology and Microbiology Section, Immune response, Immunity

Received: June 14, 2017 Accepted: August 09, 2017 Published: August 27, 2017

Abstract

IFITM3 is involved in cell adhesion, apoptosis, immune, and antivirus activity. Furthermore, IFITM3 gene has been considered as a preferential marker for inflammatory diseases, and positive correlation to pathological grades. Therefore, we assumed that IFITM3 was regulated by different signal pathways. To better understand IFITM3 function in inflammatory response, we cloned swine IFITM3 gene, and detected IFITM3 distribution in tissues, as well as characterized this gene. Results indicated that the length of swine IFITM3 gene was 438 bp, encoding 145 amino acids. IFITM3 gene expression abundance was higher in spleen and lungs. Moreover, we next constructed the eukaryotic expression vector PBIFM3 and transfected into PK15 cells, finally obtained swine IFITM3 gene stable expression cell line. Meanwhile, we explored the effects of LPS on swine IFITM3 expression. Results showed that LPS increased IFITM3 mRNA abundance and exhibited time-dependent effect for LPS treatment. To further demonstrate the mechanism that IFITM3 regulated type I IFNs production, we also detected the important molecules expression of TLR4 signaling pathway. In transfected and non-transfected IFITM3 PK15 cells, LPS exacerbated the relative expression of TLR4-NFκB signaling molecules. However, the IFITM3 overexpression suppressed the inflammatory development of PK15 cells. In conclusion, these data indicated that the overexpression of swine IFITM3 could decrease the inflammatory response through TLR4 signaling pathway, and participate in type I interferon production. These findings may lead to an improved understanding of the biological function of IFITM3 in inflammation.


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