Oncotarget

Research Papers:

SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway

Suxia Yang _, Junwei Zhang, Shiying Wang, Xinxin Zhao and Jun Shi

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Oncotarget. 2017; 8:91185-91198. https://doi.org/10.18632/oncotarget.20434

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Abstract

Suxia Yang1,*, Junwei Zhang1,*, Shiying Wang1, Xinxin Zhao1 and Jun Shi1

1Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, 475000, China

*These authors have contributed equally to this work

Correspondence to:

Suxia Yang, email: suxiaa1@163.com

Keywords: diabetic nephropathy, SOCS2, renal injury, TLR4/NF-Tβ pathway, inflammatory cytokines

Received: December 08, 2016    Accepted: July 18, 2017    Published: August 24, 2017

ABSTRACT

Suppressor of cytokine signaling 2 (SOCS2) was reported to be involved in the development of Diabetic Nephropathy (DN). However, its underlying mechanism remains undefined. Western blot was carried out to determine the expressions of SOCS2, Toll-like receptors 4 (TLR4) and nuclear factor kappa B (NF-κB) pathway-related proteins in DN patients, streptozotocin (STZ)-induced DN rats and high glucose (HG)-stimulated podocytes. The effects of SOCS2 overexpression on renal injury, the inflammatory cytokines production, renal pathological changes, apoptosis and the TLR4/NF-κB pathway in DN rats or HG-stimulated podocytes were investigated. TLR4 antagonist TAK-242 and NF-κB inhibitor PDTC were used to confirm the functional mechanism of SOCS2 overexpression in HG-stimulated podocytes. SOCS2 was down-regulated, while TLR4 and NF-κB were up-regulated in renal tissues of DN patients and DN rats. Ad-SOCS2 infection alleviated STZ-induced renal injury and pathological changes and inhibited STZ-induced IL-6, IL-1β and MCP-1 generation and activation of the TLR4/NF-κB pathway in DN rats. SOCS2 overexpression attenuated apoptosis, suppressed the inflammatory cytokines expression, and inactivated the TLR4/NF-κB pathway in HG-stimulated podocytes. Suppression of the TLR4/NF-κB pathway enhanced the inhibitory effect of SOCS2 overexpression on apoptosis and inflammatory cytokines expressions in HG-stimulated podocytes. SOCS2 overexpression alleviated the development of DN by inhibiting the TLR4/NF-κB pathway, contributing to developing new therapeutic strategies against DN.


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