Oncotarget

Research Papers:

The stress phenotype makes cancer cells addicted to CDT2, a substrate receptor of the CRL4 ubiquitin ligase

Martina Olivero, Daniela Dettori, Sabrina Arena, Davide Zecchin, Erica Lantelme and Maria Flavia Di Renzo _

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Oncotarget. 2014; 5:5992-6002. https://doi.org/10.18632/oncotarget.2042

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Abstract

Martina Olivero1,2, Daniela Dettori1,2,3, Sabrina Arena1,2, Davide Zecchin1,2,4, Erica Lantelme1,2,5 and Maria Flavia Di Renzo1,2

1 Department of Oncology, University of Torino, Candiolo, Torino, Italy

2 Candiolo Cancer Institute – FPO IRCCS, Candiolo, Torino, Italy

3 present address: HUGEF, Human Genetics Foundation, Torino, Italy

4 present address: Signal Transduction Laboratory, Cancer Research UK London Research Institute, London U.K.

5 present address: Washington University in St. Louis, St. Louis, MO

Correspondence:

Maria Flavia Di Renzo , email:

Keywords: CDT2, Ubiquitin ligase, CDT1, cancer

Received: March 17, 2014 Accepted: May 29, 2014 Published: May 30, 2014

Abstract

CDT2/L2DTL/RAMP is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. Here we show that CDT2 depletion was alone able to induce the apoptotic death in 12/12 human cancer cell lines from different tissues, regardless of the mutation profile and CDT2 expression level. Cell death was associated to rereplication and to loss of CDT1 degradation. Conversely, CDT2 depletion did not affect non-transformed human cells, such as immortalized kidney, lung and breast cell lines, and primary cultures of endothelial cells and osteoblasts. The ectopic over-expression of an activated oncogene, such as the mutation-activated RAS or the amplified MET in non-transformed immortalized breast cell lines and primary human osteoblasts, respectively, made cells transformed in vitro, tumorigenic in vivo, and susceptible to CDT2 loss. The widespread effect of CDT2 depletion in different cancer cells suggests that CDT2 is not in a synthetic lethal interaction to a single specific pathway. CDT2 likely is a non-oncogene to which transformed cells become addicted because of their enhanced cellular stress, such as replicative stress and DNA damage.


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