Research Papers: Pathology:

PKCθ utility in diagnosing c-KIT/DOG-1 double negative gastrointestinal stromal tumors

Attila Kövecsi, Ioan Jung, Zoltan Szentirmay, Tivadar Bara, Tivadar Bara Jr, Daniel Popa and Simona Gurzu _

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Oncotarget. 2017; 8:55950-55957. https://doi.org/10.18632/oncotarget.19116

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Attila Kövecsi1,2,*, Ioan Jung1,*, Zoltan Szentirmay3, Tivadar Bara4, Tivadar Bara Jr4, Daniel Popa4 and Simona Gurzu1,2,5

1 Department of Pathology, University of Medicine and Pharmacy, Tirgu Mures, Romania

2 Department of Pathology, Clinical County Emergency Hospital, Tirgu Mures, Romania

3 Department of Molecular Pathology, National Institute of Onology, Budapest, Hungary

4 Department of Surgery, University of Medicine and Pharmacy, Tirgu Mures, Romania

5 Department of Pathology, CCAMF-Research Center, Tirgu Mures, Romania

* These authors have equal contribution to the paper

Correspondence to:

Simona Gurzu, email:

Keywords: c-theta, protein kinase, GIST, diagnosis, CD117, Pathology Section

Received: April 04, 2017 Accepted: June 04, 2017 Published: July 08, 2017


Background: The aim of this study was to evaluate the diagnosis value of an immunohistochemical (IHC) panel of three antibodies for the diagnosis of gastrointestinal stromal tumors (GISTs).

Material and methods: In 80 consecutive GISTs without lymph node metastases, the IHC examinations were performed using the antibodies CD117 (c-KIT), DOG-1 and c-theta (PKCθ) protein. The diagnostic value of PKCθ in c-KIT/DOG-1 negative GISTs has been explored in fewer than 10 Medline-indexed papers.

Results: The c-KIT, PKCθ and DOG-1 positivity was noted in 92.50% (n = 74), 90% (n = 72) and 76.25% (n = 61) of the cases, respectively. All of the C-KIT negative cases (n = 6) were also DOG-1 negative but displayed PKCθ positivity. All of the DOG-1 positive cases (n = 61) also expressed c-KIT. No correlation between the examined markers and clinicopathological parameters was noted.

Conclusions: The PKCθ sensitivity is similar to c-KIT and superior to DOG-1 sensitivity. All of the c-KIT/DOG-1 negative GISTs seem to express PKCθ. For a proper diagnosis of GIST, the c-KIT/DOG-1/PKCθ panel should be used, with possible therapeutic but not prognostic value.

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