Oncotarget

Research Papers:

CyclinD1 and p57kip2 as biomarkers in differentiation, metastasis and prognosis of gastric cardia adenocarcinoma

Yi Ru, Xiao-Jie Chen, Zhi-Wei Zhao, Peng-Fei Zhang, Shuai-Hao Feng, Qiang Gao, She-Gan Gao and Xiao-Shan Feng _

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:73860-73870. https://doi.org/10.18632/oncotarget.18008

Metrics: PDF 837 views  |   HTML 1511 views  |   ?  


Abstract

Yi Ru1,*, Xiao-Jie Chen2,*, Zhi-Wei Zhao1, Peng-Fei Zhang1, Shuai-Hao Feng1, Qiang Gao1, She-Gan Gao1,2,3,4 and Xiao-Shan Feng1,3,4

1The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology , Luoyang, Henan 471003, China

2Medical College, Henan University of Science and Technology , Luoyang, Henan 471003, China

3Henan Key Laboratory of Cancer Epigenetics, Henan University of Science and Technology , Luoyang, Henan 471003, China

4Cancer Institute, Henan University of Science and Technology , Luoyang, Henan 471003, China

*These authors contributed equally to this work and co-first authors

Correspondence to:

Xiao-Shan Feng, email: fengxiaoshan880923@163.com

Keywords: gastric cardia adenocarcinoma, p57kip2, cyclinD1, clinical stage, different degrees of differentiation

Received: February 21, 2017     Accepted: April 19, 2017     Published: May 19, 2017

ABSTRACT

Objective: This study aims to investigate the expression and significance of p57kip2 and cyclinD1 in gastric cardia adenocarcinoma (GCA). p57kip2 is a negative regulator in the cell cycle. On the contrary, cyclinD1 is a positive regulator of cell cycle progression. Methods: Thirty-two cases of GCA tissues and adjacent non-cancerous tissues were collected for this study. Immunohistochemistry and fluorescence qualitative PCR was used to determine the level of p57kip2 and cyclinD1 in GCA and its adjacent non-cancerous tissues. Furthermore, the correlation between the mRNA/protein and GCA clinical pathologic parameters were analyzed, and the ralationship of p57kip2 and cyclinD1 in GCA were also evaluated. Results: The expression of p57kip2 significantly lower in GCA (P = 0.036), and there was a significant correlation in the different degrees of differentiation (P < 0.05). Furthermore, median survival time was 41 months for patients with high mRNA expression of p57kip2. This was longer compared to patients with low mRNA expression of P57kip2 (37 months, X2 = 4.788, P = 0.029).The expression of cyclinD1 was significantly higher in GCA(P = 0.002), and was significant correlated to clinical stage(P<0.05). Median survival time was 34 months in patients with high mRNA expression of cyclinD1, which was shorter than in patients with low expression of cyclinD1 mRNA (41 months, X2 = 4.071, P = 0.044). The protein expression of p57kip2 was not correlated to the protein expression of cyclinD1 (P = 0.55). Conclusion: The expression of p57kip2 and cyclinD1 are likely to suppress or promote the tumorigenesis and progression of GCA.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 18008