Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells
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Li-Chun Zheng1,2,*, Xiao-Qing Wang2,*, Kun Lu3,*, Xiao-Ling Deng3, Cheng-Wei Zhang4, Hong Luo2, Xu-Dong Xu2, Xiao-Man Chen2, Lu Yan5, Yi-Qing Wang2 and Song-Lin Shi3
1Medical College of Xiamen University, Jinshan Community Health Service Center, Xiamen Traditional Chinese Medical Hospital, Xiamen 361000, P.R. China
2Xiamen Heart Center, Medical College of Xiamen University, Xiamen 361000, P.R. China
3Department of Basic Medicine, Medical College of Xiamen University, Cancer Research Center of Xiamen University, Xiamen 361102, P.R. China
4Department of Cardiology, Affiliated Dongnan Hospital of Xiamen University, Zhangzhou 363000, P.R. China
5Department of Basic Medicine, Medical College of Xiamen University, Xiamen 361102, P.R. China
*These authors have contributed equally to this work
Yi-Qing Wang, email: [email protected]
Song-Lin Shi, email: [email protected]
Keywords: ephrin-B2, proliferation, migration, human umbilical vein endothelial cell, proteomic analysis
Received: December 09, 2016 Accepted: April 03, 2017 Published: April 20, 2017
Tumor growth and metastasis are angiogenesis dependent. Angiogenic growth involves endothelial cell proliferation, migration, and invasion. Ephrin-B2 is a ligand for Eph receptor tyrosine kinases and is an important mediator in vascular endothelial growth factor-mediated angiogenesis. However, research offer controversial information regarding effects of ephrin-B2 on vascular endothelial cells. In this paper, proteome analyses showed that ephrin-B2/Fc significantly activates multiple signaling pathways related to cell proliferation, survival, and migration and suppresses apoptosis and cell death. Cytological experiments further confirm that ephrin-B2/Fc stimulates endothelial cell proliferation, triggers dose-dependent migration, and suppresses cell apoptosis. Results demonstrate that soluble dose-dependent ephrinB2 can promote proliferation and migration and inhibit apoptosis of human umbilical vein endothelial cells. These results also suggest that ephrinB2 prevents ischemic disease and can potentially be a new therapeutic target for treating angiogenesis-related diseases and tumors.
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