Salmonella mediated the hemagglutinating virus of Japan-envelope transfer suppresses tumor growth
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Che-Hsin Lee1,2, Tomoyuki Nishikawa3, Yasufumi Kaneda3
1Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan
2Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan
3Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, Osaka, Japan
Che-Hsin Lee, email: [email protected]
Yasufumi Kaneda, email: [email protected]
Keywords: Salmonella, targeted therapy, polymer, hemagglutinating virus of Japan-envelope
Received: December 08, 2016 Accepted: March 30, 2017 Published: April 11, 2017
Salmonella can target to tumor microenvironments after systemic treatment. The hemagglutinating virus of Japan-envelope (HVJ-E) induced apoptosis in tumor cells without toxicity in normal cells. Current HVJ-E therapeutic strategies, aimed at using HVJ-E for intratumor treatment, have shown great promise in animal models but have achieved only limited systemic treatment. The purpose of this study was to investigate the modulation of the anti-tumor efficiency of HVJ-E by coating the particles with poly (allylamine hydrochloride) (PAH), designated as P-HVJ-E. Treatment with P-HVJ-E resulted in decreased hemagglutinating activity and maintained tumor cell-selective apoptosis and anti-tumor immunity. The use of Salmonella as a coating for P-HVJ-E (PHS) enhanced the antitumor activity and maintained the tumor-targeting activity. Treatment with PHS resulted in delayed tumor growth in tumor-bearing mice. Furthermore, a Western blot assay of the tumors revealed that HVJ-E targeted to the tumor after systemic treatment with PHS. These results indicate that Salmonella coating viral particles may provide a new approach for tumor therapy.
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