Research Papers:
Magnesium isoglycyrrhizinate shows hepatoprotective effects in a cyclophosphamideinduced model of hepatic injury
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Wenjiao Jiang1, Jingyan Liu2, Peijin Li1, Qianfeng Lu1, Xue Pei1, Yilin Sun1, Guangji Wang1, Kun Hao1
1Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China
2Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing 210009, China
Correspondence to:
Guangji Wang, email: [email protected]
Kun Hao, email: [email protected]
Keywords: magnesium isoglycyrrhizinate, cyclophosphamide, hepatic injury
Received: October 19, 2016 Accepted: March 04, 2017 Published: March 28, 2017
ABSTRACT
The purpose of the current study was to investigate the effect of Magnesium Isoglycyrrhizinate (GM) on cyclophosphamide (CP)-induced hepatic injury in vivo and in vitro. The results demonstrated that GM exerted a protective effect on CP-induced acute liver injury, as evidenced by the alleviations of hepatic pathological damage and serum transaminase activities. Meantime, GM attenuated serum and HepG2 cell supernatant levels of TNF-α, IL-6, IL-1β, SOD and MDA. Western blot results presented that GM down-regulated the expressions of the microtubule associated protein 1A/1B-light chain 3 (LC3), Lysosome associated membrane protein-1 (LAMP-1), p-phosphatidylinositol 3-kinase (PI3K), p-protein Kinase B(Akt), p-mechanistic target of rapamycin(mTOR), p-ribosomal protein S6 kinase 70 kDa (p70S6K), p-4E binding protein 1(4EBP1), p- inhibitor of NF-κB(IκB)α and p-nuclear factor kappa B(NF-κB)p65 in CP-stimulated hepatic tissue and HepG2 cells. Taken together, our results suggested that GM showed beneficial effect on CP-induced liver injury through NF-κB-mediated inflammation and PI3K/Akt/mTOR/p70S6K/4EBP1 axis-mediated autophagy in vivo and in vitro.