Oncotarget

Research Papers:

Magnesium isoglycyrrhizinate shows hepatoprotective effects in a cyclophosphamide-induced model of hepatic injury

Wenjiao Jiang, Jingyan Liu, Peijin Li, Qianfeng Lu, Xue Pei, Yilin Sun, Guangji Wang and Kun Hao _

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Oncotarget. 2017; 8:33252-33264. https://doi.org/10.18632/oncotarget.16629

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Abstract

Wenjiao Jiang1, Jingyan Liu2, Peijin Li1, Qianfeng Lu1, Xue Pei1, Yilin Sun1, Guangji Wang1, Kun Hao1

1Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China

2Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing 210009, China

Correspondence to:

Guangji Wang, email: guangjiwang@hotmail.com

Kun Hao, email: cpu_haokun@aliyun.com

Keywords: magnesium isoglycyrrhizinate, cyclophosphamide, hepatic injury

Received: October 19, 2016     Accepted: March 04, 2017     Published: March 28, 2017

ABSTRACT

The purpose of the current study was to investigate the effect of Magnesium Isoglycyrrhizinate (GM) on cyclophosphamide (CP)-induced hepatic injury in vivo and in vitro. The results demonstrated that GM exerted a protective effect on CP-induced acute liver injury, as evidenced by the alleviations of hepatic pathological damage and serum transaminase activities. Meantime, GM attenuated serum and HepG2 cell supernatant levels of TNF-α, IL-6, IL-1β, SOD and MDA. Western blot results presented that GM down-regulated the expressions of the microtubule associated protein 1A/1B-light chain 3 (LC3), Lysosome associated membrane protein-1 (LAMP-1), p-phosphatidylinositol 3-kinase (PI3K), p-protein Kinase B(Akt), p-mechanistic target of rapamycin(mTOR), p-ribosomal protein S6 kinase 70 kDa (p70S6K), p-4E binding protein 1(4EBP1), p- inhibitor of NF-κB(IκB)α and p-nuclear factor kappa B(NF-κB)p65 in CP-stimulated hepatic tissue and HepG2 cells. Taken together, our results suggested that GM showed beneficial effect on CP-induced liver injury through NF-κB-mediated inflammation and PI3K/Akt/mTOR/p70S6K/4EBP1 axis-mediated autophagy in vivo and in vitro.


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