Oncotarget

Research Papers:

Comparison of the tumor cell secretome and patient sera for an accurate serum-based diagnosis of pancreatic ductal adenocarcinoma

Shakhawan Mustafa, Longqiang Pan, Aseel Marzoq, Malak Fawaz, Laureen Sander, Felix Rückert, Andrea Schrenk, Christina Hartl, Rico Uhler, Adem Yildirim, Oliver Strobel, Thilo Hackert, Nathalia Giese, Markus W. Büchler, Jörg D. Hoheisel and Mohamed Saiel Saeed Alhamdani _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:11963-11976. https://doi.org/10.18632/oncotarget.14449

Metrics: PDF 2204 views  |   HTML 2273 views  |   ?  


Abstract

Shakhawan Mustafa1,2,*, Longqiang Pan1,*, Aseel Marzoq1, Malak Fawaz1, Laureen Sander1, Felix Rückert3, Andrea Schrenk3, Christina Hartl1, Rico Uhler1, Adem Yildirim1, Oliver Strobel4, Thilo Hackert4, Nathalia Giese4, Markus W. Büchler4, Jörg D. Hoheisel1,*, Mohamed Saiel Saeed Alhamdani1,*

1Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany

2Kurdistan Institution for Strategic Studies and Scientific Research, Kurdistan Region, Iraq

3Chirurgische Klinik, Universitätsmedizin Mannheim, Theodor-Kutzer-Ufer 1-3, Mannheim, Germany

4Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany

*These authors have contributed equally to this work

Correspondence to:

Mohamed Saiel Saeed Alhamdani, email: m.alhamdani@dkfz.de

Keywords: secretome, pancreatic cancer, biomarkers, cell lines, antibody microarray

Received: February 09, 2016     Accepted: December 25, 2016     Published: January 2, 2017

ABSTRACT

Pancreatic cancer is the currently most lethal malignancy. Toward an accurate diagnosis of the disease in body liquids, we studied the protein composition of the secretomes of 16 primary and established cell lines of pancreatic ductal adenocarcinoma (PDAC). Compared to the secretome of non-tumorous cells, 112 proteins exhibited significantly different abundances. Functionally, the proteins were associated with PDAC features, such as decreased apoptosis, better cell survival and immune cell regulation. The result was compared to profiles obtained from 164 serum samples from two independent cohorts – a training and a test set – of patients with PDAC or chronic pancreatitis and healthy donors. Eight of the 112 secretome proteins exhibited similar variations in their abundance in the serum profile specific for PDAC patients, which was composed of altogether 189 proteins. The 8 markers shared by secretome and serum yielded a 95.1% accuracy of distinguishing PDAC from healthy in a Receiver Operating Characteristic curve analysis, while any number of serum-only markers produced substantially less accurate results. Utility of the identified markers was confirmed by classical enzyme linked immunosorbent assays (ELISAs). The study highlights the value of cell secretome analysis as a means of defining reliable serum biomarkers.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 14449