The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer
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Liu Enyu1,*, Wang Na2,*, Zhao Chuanzong1, Wang Ben1, Wu Xiaojuan3, Wang Yan3, Li Zequn1,4, Hong Jianguo1, Wang Jiayong1, Liang Benjia1,4, Peng Cheng1, Zhu Min1, Zhang Zongli1
1Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China
2Department of Dermatology, Shandong Provinical Institute of dermatology and venereology, Jinan 250012, Shandong, P.R. China
3Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong, P.R. China
4Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Public Health, Jinan 250012, Shandong, P.R. China
*The First two authors have contributed equally to this work
Liu Enyu, email: [email protected]
Zhang Zongli, email: [email protected]
Zhu Min, email: [email protected]
Keywords: gallbladder cancer, integrin β6, pStat-3, immunohistochemistry, tumorigenesis
Received: April 28, 2016 Accepted: November 01, 2016 Published: January 02, 2017
Background: Both phosphorylated signal transducer and activator of transcription 3(pStat-3) and integrin αvβ6 can play vital role in the development and progression of cancer. However, little is known about their expression correlation and clinical significance in gallbladder cancer(GBC).
Objective: The aim of our present study was to investigate the expression of pStat-3 and integrin αvβ6, two proteins’ correlation and their clinical significance in GBC tissues.
Results: The expression of pStat-3 and integrin αvβ6 were both significantly associated with T stage, lymph node metastasis status, TNM stage (P=0.008, P=0.000, P=0.000 and P=0.036, P=0.001,P=0.000,respectively). IHC and Western blot showed their expressions in GBC tissues were higher than that in paraneoplastic tissues. Moderate positive correlation existed between the two proteins (r =0.349, P <0.001). The survival analysis by Kaplan-Meier and Cox regression model showed that GBC patients with pStat-3 or integrin αvβ6 positive expression had a significantly poorer 2-year survival rate (P = 0.002 and 0.000, the log-rank test, respectively), and either marker could act as unfavorable independent prognostic factors(RR=1.907, P=0.021 and RR=2.046, P=0.038).
Materials and Methods: The expression levels of pStat-3 and integrin αvβ6 were analyzed in GBC cancerous and paraneoplastic tissues of 97 cases via immunohistochemistry(IHC) and further validated by western blot method. Besides, SPSS software was used to observe their clinical significance as well as the two proteins’ correlation.
Conclusion: pStat-3 and integrin αvβ6 were indicators of tumor’s progression and poor prognosis of patients with GBC. And the further study involving them may provide a helpful therapeutic target in prevention and treatment of GBC patients.
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