Oncotarget

Research Papers:

mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27

Zhuoying Feng, Luping Zhang, Junchen Zhou, Shuai Zhou, Li li, Xuyan Guo, Guoying Feng, Ze Ma, Wenhua Huang and Fei Huang _

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Oncotarget. 2017; 8:6304-6318. https://doi.org/10.18632/oncotarget.13850

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Abstract

Zhuoying Feng1,*, Luping Zhang1,*, Junchen Zhou1, Shuai Zhou1, Li li1, Xuyan Guo1, Guoying Feng1, Ze Ma1, Wenhua Huang2, Fei Huang1

1Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, Laishan District, Shandong Province, 264003,China

2Institute of Clinical Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China

*These authors contributed to this work equally and should be considered as co-first authors

Correspondence to:

Fei Huang, email: hfei22518@163.com

Wenhua Huang, email: huangwenhua2009@139.com

Keywords: gliomas, mir-218-2, CDC27, proliferation, invasion

Received: December 28, 2015    Accepted: November 02, 2016    Published: December 10, 2016

ABSTRACT

Glioma has become a significant global health problem with substantial morbidity and mortality, underscoring the importance of elucidating its underlying molecular mechanisms. Recent studies have identified mir-218 as an anti-oncogene; however, the specific functions of mir-218-1 and mir-218-2 remain unknown, especially the latter. The objective of this study was to further investigate the role of mir-218-2 in glioma. Our results indicated that mir-218-2 is highly overexpressed in glioma. Furthermore, we showed that mir-218-2 is positively correlated with the growth, invasion, migration, and drug susceptibility (to β-lapachone) of glioma cells. In vitro, the overexpression of mir-218-2 promoted glioma cell proliferation, invasion, and migration. In addition, the overexpression of mir-218-2 in vivo was found to increase glioma tumor growth. Accordingly, the inhibition of mir-218-2 resulted in the opposite effects. Cell division cycle 27 (CDC27), the downstream target of mir-218-2, is involved in the regulation of glioma cells. Our results indicate that the overexpression of CDC27 counteracted the function of mir-218-2 in glioma cells. These novel findings provide new insight in the application of mir-218-2 as a potential glioma treatment.


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