Oncotarget

Research Papers:

Curcumin-carrying nanoparticles prevent ischemia-reperfusion injury in human renal cells

Yong Xu _, Ning Hu, Wei Jiang, Hong-Fang Yuan and Dong-Hui Zheng

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Oncotarget. 2016; 7:87390-87401. https://doi.org/10.18632/oncotarget.13626

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Abstract

Yong Xu1,*, Ning Hu2,*, Wei Jiang1,*, Hong-Fang Yuan3, Dong-Hui Zheng1

1Department of Nephrology, The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, Huai'an 223002, China

2Department of Nephrology, The First People's Hospital of Jingmen, Jingmen, Hubei 448000, China

3Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

*Co-first authors

Correspondence to:

Dong-Hui Zheng, email: [email protected]

Hong-Fang Yuan, email: [email protected]

Keywords: Curcumin, nanoparticles, ischemia-reperfusion injury

Received: August 24, 2016     Accepted: November 08, 2016     Published: November 25, 2016

ABSTRACT

Renal ischemia-reperfusion injury (IRI) is a major complication in clinical practice. However, despite its frequency, effective preventive/treatment strategies for this condition are scarce. Curcumin possesses antioxidant properties and is a promising potential protective agent against renal IRI, but its poor water solubility restricts its application. In this study, we constructed curcumin-carrying distearoylphosphatidylethanolamine-polyethylene glycol nanoparticles (Cur-NPs), and their effect on HK-2 cells exposed to IRI was examined in vitro. Curcumin encapsulated in NPs demonstrated improved water solubility and slowed release. Compared with the IRI and Curcumin groups, Cur-NP groups displayed significantly improved cell viability, downregulated protein expression levels of caspase-3 and Bax, upregulated expression of Bcl-2 protein, increased antioxidant superoxide dismutase level, and reduced apoptotic rate, reactive oxygen species level, and malondialdehyde content. Results clearly showed that Cur-NPs demonstrated good water solubility and slow release, as well as exerted protective effects against oxidative stress in cultured HK-2 cells exposed to IRI.


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