Research Papers:

Activation of chronic toxoplasmosis by transportation stress in a mouse model

Bang Shen _, Yuan Yuan, Jianxi Cheng, Ming Pan, Ningbo Xia, Weichao Zhang, Yifan Wang, Yanqin Zhou and Junlong Zhao

PDF  |  HTML  |  How to cite

Oncotarget. 2016; 7:87351-87360. https://doi.org/10.18632/oncotarget.13568

Metrics: PDF 1642 views  |   HTML 2181 views  |   ?  


Bang Shen1, Yuan Yuan1, Jianxi Cheng1, Ming Pan1, Ningbo Xia1, Weichao Zhang1, Yifan Wang1, Yanqin Zhou1, Junlong Zhao1,2

1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China

2Hubei Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, Hubei, PR China

Correspondence to:

Junlong Zhao, email: [email protected]

Keywords: Toxoplasma gondii, chronic infection, bradyzoite, reactivation, transportation stress

Received: September 28, 2016     Accepted: November 02, 2016     Published: November 24, 2016


Toxoplasma gondii is an obligate intracellular parasite infecting 25% of the world population and enormous number of animals. It can exist in two forms in intermediate hosts: the fast replicating tachyzoites responsible for acute infection and the slowly replicating bradyzoites responsible for life-long chronic infection. The interconversion between tachyzoites and bradyzoites plays critical roles in the transmission and pathogenesis of T. gondii. However, the molecular mechanisms that govern the interconversion are largely unknown. In this study, we established a chronic infection model in mice and examined the impact of transportation stress on the status of chronic infection. Our results demonstrated that, treating chronically infected mice with conditions mimicking transportation stress reduced the levels of several key cytokines that restrict the infection at chronic stage. Increased expression of the tachyzoite specific gene SAG1 (surface antigen 1) was detected in brain cysts of stress treated mice, indicating activation and conversion of bradyzoites to tachyzoites. Using this model, we identified fifteen toxoplasmic proteins that had significant abundance changes during stress induced cysts reactivation. These proteins serve as a basis for further investigation of the mechanisms governing bradyzoite conversion.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 13568