Research Papers:

Elevated expression of STIM1 is involved in lung tumorigenesis

Yadong Wang _, Haiyu Wang, Li Li, Jiangmin Li, Teng Pan, Ding Zhang and Haiyan Yang

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Oncotarget. 2016; 7:86584-86593. https://doi.org/10.18632/oncotarget.13359

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Yadong Wang1,2, Haiyu Wang1, Li Li1, Jiangmin Li1, Teng Pan3, Ding Zhang1, Haiyan Yang3

1Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China

2Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, China

3Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, China

Correspondence to:

Yadong Wang, email: [email protected], [email protected]

Haiyan Yang, email: [email protected]

Keywords: STIM1, A549 cell, lung cancer, tumorigenicity, cell cycle

Received: September 14, 2016    Accepted: October 29, 2016    Published: November 15, 2016


This study aimed to address the potential role of STIM1 (stromal interaction molecule 1) in lung tumorigenesis. Colony formation in soft agar assay and tumorigenicity in nude mice assay were conducted. Western blot, immunohistochemistry and quantitative real-time polymerase chain reaction were used to measure the STIM1 expression. The distribution of cell cycle was detected by flow cytometry assay. Our results showed that the expression of STIM1 mRNA was significantly higher in human lung tumors than that in adjacent non-neoplastic lung tissues. Significantly increased expression of STIM1 mRNA and protein was observed in 16HBE-benzo(a)pyrene (BaP) cells and in BaP-treated mice lung tissues compared with 16HBE-control cells and the control group, respectively. Silencing STIM1 inhibited the proliferation and colony formation of A549 cells in in vitro experiments, attenuated the growth of tumor xenografts of A549 cells in in vivo experiments and induced the arrest of cell cycle in the G1 phase. The markedly decreased expression of cyclin D1 protein was observed in A549-shRNA-STIM1 cells as compared to A549-shRNA-control cells. The markedly increased expression of p21 protein was observed in A549-shRNA-STIM1 cells as compared to A549-shRNA-control cells. The expression levels of β-catenin and TGIF proteins were lower in A549-shRNA-STIM1 cells than those in A549-shRNA-control cells. In conclusion, this study indicated that the elevated expression of STIM1 might be involved in lung tumorigenesis.

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