Oncotarget

Research Papers:

Fibroblast growth factor signalling induces loss of progesterone receptor in breast cancer cells

Dominika Piasecka, Kamila Kitowska, Dominika Czaplinska, Kamil Mieczkowski, Magdalena Mieszkowska, Lukasz Turczyk, Andrzej C. Skladanowski, Anna J. Zaczek, Wojciech Biernat, Radzislaw Kordek, Hanna M. Romanska and Rafal Sadej _

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Oncotarget. 2016; 7:86011-86025. https://doi.org/10.18632/oncotarget.13322

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Abstract

Dominika Piasecka1,4, Kamila Kitowska1, Dominika Czaplinska2, Kamil Mieczkowski1, Magdalena Mieszkowska1, Lukasz Turczyk1, Andrzej C. Skladanowski1, Anna J. Zaczek2, Wojciech Biernat3, Radzislaw Kordek4, Hanna M. Romanska4, Rafal Sadej1

1Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Poland

2Department of Cell Biology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Poland

3Department of Pathomorphology, Medical University of Gdansk, Poland

4Department of Pathology, Medical University of Lodz, Poland

Correspondence to:

Rafal Sadej, email: [email protected]

Hanna M. Romanska, email: [email protected]

Keywords: progesterone receptor, FGFR2, breast cancer

Received: April 12, 2016     Accepted: November 07, 2016     Published: November 12, 2016

ABSTRACT

We have recently demonstrated that, fibroblast growth factor 2 (FGFR2), signalling via ribosomal S6 kinase 2 (RSK2), promotes progression of breast cancer (BCa). Loss of progesterone receptor (PR), whose activity in BCa cells can be stimulated by growth factor receptors (GFRs), is associated with poor patient outcome. Here we showed that FGF7/FGFR2 triggered phosphorylation of PR at Ser294, PR ubiquitination and subsequent receptor`s degradation via the 26S proteasome pathway in BCa cells. We further demonstrated that RSK2 mediated FGF7/FGFR2-induced PR downregulation. In addition, a strong synergistic effect of FGF7 and progesterone (Pg), reflected in the enhanced anchorage-independent growth and cell migration, was observed. Analysis of clinical material demonstrated that expression of PR inversely correlated with activated RSK (RSK-P) (p = 0.016). Patients with RSK-P(+)/PR(–) tumours had 3.629-fold higher risk of recurrence (p = 0.002), when compared with the rest of the cohort. Moreover, RSK-P(+)/PR(–) phenotype was shown as an independent prognostic factor (p = 0.006). These results indicate that the FGF7/FGFR2-RSK2 axis promotes PR turnover and activity, which may sensitize BCa cells to stromal stimuli and contribute to the progression toward steroid hormone negative BCa.


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