Oncotarget

Research Papers:

miR-186 affects the proliferation, invasion and migration of human gastric cancer by inhibition of Twist1

Chunhong Cao _, Deguang Sun, Liang Zhang and Lei Song

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Oncotarget. 2016; 7:79956-79963. https://doi.org/10.18632/oncotarget.13182

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Abstract

Chunhong Cao1,*, Deguang Sun1,*, Liang Zhang1, Lei Song1

1Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116027 Liaoning, China

*These authors have contributed equally to this work as the co-first author

Correspondence to:

Liang Zhang, email: zhangliangdlmu@163.com

Lei Song, email: songleidlmu@163.com

Keywords: miR-186, Twist1, GC

Received: September 12, 2016     Accepted: October 17, 2016     Published: November 07, 2016

ABSTRACT

Recent evidence shows that miRNAs are dysregulated in a variety of cancers including gastric cancer (GC), and emerging as key oncogenes or tumor suppressors. In this study, qRT-PCR was used to analyze the expression of miR-186 in GC tissues and adjacent non-cancerous tissues, and then more in-vitro experiments were used to investigate the role of miR-186 in GC cells. Here, we identified miR-186 was generally down-regulated in GC tissues; however, Twist1 was generally up-regulated in GC tissues. Moreover, miR-186 and Twist1 were associated with larger tumor size and advanced clinical stage of GC. In-vitro experiments demonstrated that ectopic overexpression of miR-186 inhibited GC cell proliferation, invasion and migration; however, inhibited expression of miR-186 enhanced cell proliferation, invasion and migration. Furthermore, the luciferase reporter assay demonstrated Twist1 as a direct target of miR-186. Finally, over-expression of Twist1 abrogated inhibitory impact of miR-186 on cell proliferation, invasion and migration. In conclusion, miR-186 affects the proliferation, invasion and migration of human gastric cancer by inhibition of Twist1, and could be a tumor suppressor in GC development. Thus, miR-186 may be served as a candidate prognostic biomarker and target for new therapies in human gastric cancer.


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