Research Papers: Immunology:

Activation and selective IL-17 response of human Vγ9Vδ2 T lymphocytes by TLR-activated plasmacytoid dendritic cells

Elena Lo Presti, Nadia Caccamo, Valentina Orlando, Francesco Dieli and Serena Meraviglia _

PDF  |  HTML  |  How to cite

Oncotarget. 2016; 7:60896-60905. https://doi.org/10.18632/oncotarget.11755

Metrics: PDF 2022 views  |   HTML 2833 views  |   ?  


Elena Lo Presti1,2, Nadia Caccamo1,2, Valentina Orlando1,2, Francesco Dieli1,2 and Serena Meraviglia1,2

1 Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy

2 Department of Biopathology and Medical Biotechnologies (DIBIMED), University of Palermo, Palermo, Italy

Correspondence to:

Serena Meraviglia, email:

Keywords: γδ T cells, plasmacytoid dendritic cells, IL-17, TLR activation, proliferation, Immunology and Microbiology Section, Immune response, Immunity

Received: July 20, 2016 Accepted: August 02, 2016 Published:August 31, 2016


Vγ9Vδ2 T cells and plasmacytoid dendritic cells (pDCs) are two distinct cell types of innate immunity that participate in early phases of immune response. We investigated whether a close functional relationship exists between these two cell populations using an in vitro co-culture in a human system.

pDCs that had been activated by IL-3 and the TLR9 ligand CpG induced substantial activation of Vγ9Vδ2 T cells upon co-culture, which was cell-to-cell contact dependent, as demonstrated in transwell experiments, but that did not involve any of the costimulatory molecules potentially expressed by pDCs or Vγ9V2 T cells, such as ICOS-L, OX40 and CD40L. Activated pDCs selectively induced IL-17, but not IFN-γ, responses of Vγ9Vδ2T cells, which was dominant over the antigen-induced response, and this was associated with the expansion of memory (both central and effector memory) subsets of Vγ9Vδ2 T cells.

Overall, our results provide a further piece of information on the complex relationship between these two populations of cells with innate immunity features during inflammatory responses.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 11755