Research Papers: Immunology:
Activation and selective IL17 response of human Vγ9Vδ2 T lymphocytes by TLRactivated plasmacytoid dendritic cells
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Elena Lo Presti1,2, Nadia Caccamo1,2, Valentina Orlando1,2, Francesco Dieli1,2 and Serena Meraviglia1,2
1 Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy
2 Department of Biopathology and Medical Biotechnologies (DIBIMED), University of Palermo, Palermo, Italy
Correspondence to:
Serena Meraviglia, email:
Keywords: γδ T cells, plasmacytoid dendritic cells, IL-17, TLR activation, proliferation, Immunology and Microbiology Section, Immune response, Immunity
Received: July 20, 2016 Accepted: August 02, 2016 Published:August 31, 2016
Abstract
Vγ9Vδ2 T cells and plasmacytoid dendritic cells (pDCs) are two distinct cell types of innate immunity that participate in early phases of immune response. We investigated whether a close functional relationship exists between these two cell populations using an in vitro co-culture in a human system.
pDCs that had been activated by IL-3 and the TLR9 ligand CpG induced substantial activation of Vγ9Vδ2 T cells upon co-culture, which was cell-to-cell contact dependent, as demonstrated in transwell experiments, but that did not involve any of the costimulatory molecules potentially expressed by pDCs or Vγ9V2 T cells, such as ICOS-L, OX40 and CD40L. Activated pDCs selectively induced IL-17, but not IFN-γ, responses of Vγ9Vδ2T cells, which was dominant over the antigen-induced response, and this was associated with the expansion of memory (both central and effector memory) subsets of Vγ9Vδ2 T cells.
Overall, our results provide a further piece of information on the complex relationship between these two populations of cells with innate immunity features during inflammatory responses.