Research Papers:

Gene status and clinicopathologic characteristics of lung adenocarcinomas with mediastinal lymph node metastasis

Shumeng Zhang, Bing Yan, Jing Zheng, Jing Zhao and Jianying Zhou _

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Oncotarget. 2016; 7:63758-63766. https://doi.org/10.18632/oncotarget.11494

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Shumeng Zhang1, Bing Yan1, Jing Zheng1, Jing Zhao2, Jianying Zhou1

1Department of Respiratory Disease, Thoracic Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

2Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

Correspondence to:

Jianying Zhou, email: [email protected]

Keywords: adenocarcinoma of the lung, EGFR, ALK, ROS1, RET

Received: March 24, 2016    Accepted: August 09, 2016    Published: August 22, 2016


Lung cancer with mediastinal lymph node metastasis is more likely to develop recurrence and metastasis after complete resection and targeted therapy is a promising treatment strategy. We performed amplification refractory mutation system (ARMS) fluorescence quantitative PCR to detect the gene status of EGFR, ALK, ROS1 and RET in resected samples from 280 patients who were confirmed to have primary lung adenocarcinomas with N1-N2 lymph node metastasis. Of the 280 patients enrolled, the frequency of EGFR mutations, ALK fusions, ROS1 fusions, RET fusions and no mutations was 42.9%, 10.7%, 1.8%, 3.6% and 42.9%, respectively. Five patients exhibited the coexistence of the EGFR and ALK alterations. ALK, ROS1 and RET fusions were mutually exclusive. The frequency of EGFR mutation was significantly lower among patients with poor differentiation, while the rates of ALK and ROS1 fusions were the opposite. RET fusions also tended to be more prevalent in poorly differentiated patients. EGFR and ALK double positive tumors were characterized by significantly smaller size compared with those had single gene alteration. Our study comprehensively analyzed the distinct and common clinicopathologic characteristics according to genotypes of the cohort, which should help in categorizing patients for efficient screening.

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