A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
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Donglai Lv1,2, Shi-cang Yu1,2, Yi-fang Ping1,2, Haibo Wu1,2, Xilong Zhao1,2, Huarong Zhang1,2, Youhong Cui1,2, Bing Chen3,4, Xia Zhang1,2, Jianwu Dai3,4, Xiu-wu Bian1,2,*, Xiao-hong Yao1,2,*
1Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China
2Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, China
3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, School of Military Preventive Medicine, Third Military Medical University, Chongqing, China
4Institute of Genetics and Development, Chinese Academy of Sciences, Beijing, China
*These authors have contributed equally to this work
Xiao-hong Yao, email: email@example.com
Xiu-wu Bian, email: firstname.lastname@example.org
Keywords: chemosensitivity, collagen scaffold, glioma stem cells, three-dimensional culture, MGMT
Received: February 27, 2016 Accepted: June 30, 2016 Published: July 28, 2016
Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics.
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