Oncotarget

Research Papers:

Glutaredoxin 3 promotes nasopharyngeal carcinoma growth and metastasis via EGFR/Akt pathway and independent of ROS

Feng He, Lili Wei, Wenqi Luo, Zhipeng Liao, Bo Li, Xiaoying Zhou, Xue Xiao, Jingping You, Yufeng Chen, Shixing Zheng, Ping Li, Mariko Murata, Guangwu Huang and Zhe Zhang _

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Oncotarget. 2016; 7:37000-37012. https://doi.org/10.18632/oncotarget.9454

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Abstract

Feng He1,*, Lili Wei2,*, Wenqi Luo2, Zhipeng Liao1, Bo Li1, Xiaoying Zhou1, Xue Xiao1, Jingping You1, Yufeng Chen1, Shixing Zheng1, Ping Li2, Mariko Murata3, Guangwu Huang1, Zhe Zhang1

1Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China

2Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, China

3Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine, Mie, Japan

*These authors contributed equally to this work

Correspondence to:

Zhe Zhang, email: [email protected]

Keywords: glutaredoxin 3, nasopharyngeal carcinoma, EGFR, Akt

Received: January 03, 2016     Accepted: April 16, 2016     Published: May 18, 2016

ABSTRACT

Glutaredoxin 3 (GLRX3) is antioxidant enzyme, maintaining a low level of ROS, thus contributing to the survival and metastasis of several types of cancer. However, the expression and functions of GLRX3 have not been addressed in nasopharyngeal carcinoma (NPC). In this study, we found that GLRX3 was overexpressed in NPC. Knockdown of GLRX3 in NPC cell lines inhibited proliferation in vitro, tumorignesis in vivo, and colony formation. In addition, GLRX3 knockdown decreased the migration and invasion capacity of NPC cells by reversing the epithelial-mesenchymal transition (EMT). Furthermore, stabilization of GLRX3 was positively related to with epidermal growth factor receptor (EGFR) expression and negatively with ROS generation. Phosphorylation of Akt, a key downstream effector, was induced by EGFR signaling but did not rely on increasing ROS level in NPC cells. GLRX3 might be an oncoprotein in NPC, playing important roles in increasing redox reaction and activating EGFR/ Akt signals, so it may be a therapeutic target for NPC.


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