Research Papers:

Hyperosmotic treatment synergistically boost efficiency of cell-permeable peptides

Hu Wang, Ming Zhang, Fanhui Zeng and Changbai Liu _

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Oncotarget. 2016; 7:74648-74657. https://doi.org/10.18632/oncotarget.9448

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Hu Wang1,2,4, Ming Zhang1,2, Fanhui Zeng3, Changbai Liu1,2,4

1The Institute of Cell Therapy, China Three Gorges University, Yichang, China

2Medical School, Department of Pathology, Biology and Immunology, China Three Gorges University, Yichang, China

3The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, China

4Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China

Correspondence to:

Hu Wang, email: [email protected]

Changbai Liu, email: [email protected]

Keywords: cell penetrating peptide, hypertonic molecules, penetration, osmoprotectant

Received: February 25, 2016     Accepted: April 11, 2016     Published: May 18, 2016


Therapeutics delivery into cells has been hurdled due to the barrier of cytoplasmic membrane. Although cell penetrating peptide (CPP) can potentially serve as an intracellular drug delivery vehicle, the application of CPP-based delivery is limited because the unsatisfactory delivery efficiency of CPP conjugated potent cargos is challenging their applications in present. Thus, the development of strategies for enhancing the penetrating efficiency of CPP would therefore urgent need to be explored to increase the scope of potential applications. We report here the effects of glucose, sucrose and manntiol (abbreviated as GSM) combination facilitating the penetration efficiency of CPP peptide alone or CPP-GFP (green fluorescence protein) conjugation in cultured cell lines or primary cells. Moreover, osmoprotectants glycerol and glycine supplementation help cells cope with the stress from GSM combination. Thus, our present study suggests that GSM combination in the presence of osmoprotectant can work as a new strategy for CPP penetration enhancement.

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