Oncotarget

Research Papers:

MicroRNA-126-3p suppresses cell proliferation by targeting PIK3R2 in Kaposi’s sarcoma cells

Xiu-Juan Wu _, Zong-Feng Zhao, Xiao-Jing Kang, Hong-Juan Wang, Juan Zhao and Xiong-Ming Pu

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Oncotarget. 2016; 7:36614-36621. https://doi.org/10.18632/oncotarget.9311

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Abstract

Xiu-Juan Wu1, Zong-Feng Zhao2, Xiao-Jing Kang1, Hong-Juan Wang1, Juan Zhao1, Xiong-Ming Pu1

1Department of Dermatology and Venereology, People’s Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China

2Clinical Medical Research Center, People’s Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China

Correspondence to:

Xiu-Juan Wu, email: [email protected]

Xiong-Ming Pu, email: [email protected]

Keywords: miR-126-3p, suppress, target, PIK3R2, Kaposi’s sarcoma

Received: December 19, 2015     Accepted: April 25, 2016     Published: May 12, 2016

ABSTRACT

Kaposi’s sarcoma is a highly vascular tumor of lymphatic endothelial origin. Many deregulated miRNAs, including miR-126-3p, have been identified in Kaposi’s sarcoma tissues. miR-126-3p is the most highly endothelial-specific miRNA that regulates vascular integrity and angiogenesis. In this study, we aimed to determine the effect of miR-126-3p on Kaposi’s sarcoma cells through transfection of a miRNA mimic and inhibitor. Moreover, we searched the target gene (PIK3R2) of miR-126-3p using bioinformatics software and further verified PIK3R2 using luciferase reporter assays, Real-time quantitative PCR (qRT-PCR) and western blot. The results demonstrated that miR-126-3p inhibited cell proliferation, arrested cell cycle progression, induced cell apoptosis, and inhibited cell invasion of SLK cells. The bioinformatics analysis and luciferase reporter assay revealed that PIK3R2 mRNA is a direct target of miR-126-3p. Moreover, the level of expression of the PIK3R2 gene was downregulated in SLK cells transfected with miR-126-3p siRNAs. Therefore, our data demonstrated that miR-126-3p is a tumor suppressor miRNA that acts by targeting PIK3R2 in Kaposi’s sarcoma cells. These findings contribute to our understanding of the molecular mechanisms underlying Kaposi’s sarcoma.


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