Gene expression profiling of normal thyroid tissue from patients with thyroid carcinoma
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Roberto Ria1,*, Vittorio Simeon2,*, Assunta Melaccio1, Giovanna Di Meo3, Stefania Trino2, Carmela Mazzoccoli2, Ilaria Saltarella1, Aurelia Lamanuzzi1, Annalisa Morano2, Angela Gurrado3, Alessandro Pasculli3, Gaetano Lastilla4, Pellegrino Musto2, Antonia Reale1, Franco Dammacco1, Angelo Vacca1, Mario Testini3
1Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine “G. Baccelli”, Bari, BA, Italy
2Laboratory of Pre-Clinical and Traslational Research, IRCSS CROB, Rionero in Vulture, PZ, Italy
3Department of Biomedical Sciences and Human Oncology, Unit of Endocrine, Digestive and Emergency Surgery, Bari, BA, Italy
4Department of Pathology, University of Bari “Aldo Moro” Medical School, Bari, BA, Italy
*These authors have contributed equally to this work
Roberto Ria, email: [email protected]
Keywords: gene expression profile, hypoxia, microenvironment, oncogenes, thyroid cancer
Received: November 14, 2015 Accepted: March 28, 2016 Published: April 18, 2016
Gene expression profiling (GEP) of normal thyroid tissue from 43 patients with thyroid carcinoma, 6 with thyroid adenoma, 42 with multinodular goiter, and 6 with Graves-Basedow disease was carried out with the aim of achieving a better understanding of the genetic mechanisms underlying the role of normal cells surrounding the tumor in the thyroid cancer progression. Unsupervised and supervised analyses were performed to compare samples from neoplastic and non-neoplastic diseases. GEP and subsequent RT-PCR analysis identified 28 differentially expressed genes. Functional assessment revealed that they are involved in tumorigenesis and cancer progression. The distinct GEP is likely to reflect the onset and/or progression of thyroid cancer, its molecular classification, and the identification of new potential prognostic factors, thus allowing to pinpoint selective gene targets with the aim of realizing more precise preoperative diagnostic procedures and novel therapeutic approaches.
STATEMENT OF SIGNIFICANCE
This study is focused on the gene expression profiling analysis followed by RT-PCR of normal thyroid tissues from patients with neoplastic and non-neoplastic thyroid diseases. Twenty-eight genes were found to be differentially expressed in normal cells surrounding the tumor in the thyroid cancer. The genes dysregulated in normal tissue samples from patients with thyroid tumors may represent new molecular markers, useful for their diagnostic, prognostic and possibly therapeutic implications.
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