Efficient therapeutic delivery by a novel cell-permeant peptide derived from KDM4A protein for antitumor and antifibrosis
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Hu Wang1,2,3,*, Jie-Lan Ma2,*, Ying-Gui Yang1,2,*, Yang Song1,2,3,*, Jiao Wu1,2,3, Yan-Yan Qin2,3, Xue-Li Zhao2,3, Jun Wang1,4, Li-Li Zou1,2, Jiang-Feng Wu1,2, Jun-Ming Li1,4, Chang-Bai Liu1,2,3
1The Institute of Cell Therapy, China Three Gorges University, Yichang 443002, China
2Medical School, China Three Gorges University, Yichang 443002, China
3Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China
4The 1st People’s Hospital of Yichang, Yichang 443000, China
*These authors contributed equally to this work
Chang-Bai Liu, email: [email protected]
Hu Wang, email: [email protected]
Keywords: cell-penetrating peptides (CPPs), internalization, GFP, melanoma, anti-tumor
Received: February 24, 2016 Accepted: March 28, 2016 Published: April 11, 2016
Cell-penetrating peptide (CPP) based delivery have provided immense potential for the therapeutic applications, however, most of nonhuman originated CPPs carry the risk of possible cytotoxicity and immunogenicity, thus may restricting to be used. Here, we describe a novel human-derived CPP, denoted hPP10, and hPP10 has cell-penetrating properties evaluated by CellPPD web server, as well as In-Vitro and In-Vivo analysis. In vitro studies showed that hPP10-FITC was able to penetrate into various cells including primary cultured cells, likely through an endocytosis pathway. And functionalized macromolecules, such as green fluorescent protein (GFP), tumor-specific apoptosis inducer Apoptin as well as biological active enzyme GCLC (Glutamate-cysteine ligase, catalytic subunit) can be delivered by hPP10 in vitro and in vivo. Collectively, our results suggest that hPP10 provide a novel and versatile tool to deliver exogenous proteins or drugs for clinical applications as well as reprogrammed cell-based therapy.
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