Research Papers:

Altered neutrophil immunophenotypes in childhood B‑cell precursor acute lymphoblastic leukemia

Elen Oliveira, Thiago S. Bacelar, Juana Ciudad, Maria Cecília M. Ribeiro, Daniela R.N. Garcia, Lukasz Sedek, Simone F. Maia, Daniel B. Aranha, Indyara C. Machado, Arissa Ikeda, Bianca F. Baglioli, Nathalia Lopez‑Duarte, Lisandra A. C. Teixeira, Tomasz Szczepanski, Maria Luiza M. Silva, Marcelo G.P. Land, Alberto Orfao and Elaine S. Costa _

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Oncotarget. 2016; 7:24664-24676. https://doi.org/10.18632/oncotarget.8369

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Elen Oliveira1,2, Thiago S. Bacelar2, Juana Ciudad3, Maria Cecília M. Ribeiro4, Daniela R.N. Garcia1,5, Lukasz Sedek6, Simone F. Maia7, Daniel B. Aranha1, Indyara C. Machado8, Arissa Ikeda9, Bianca F. Baglioli10, Nathalia Lopez-Duarte2, Lisandra A. C. Teixeira1,2, Tomasz Szczepanski6, Maria Luiza M. Silva5, Marcelo G.P. Land1,2, Alberto Orfao3,*, Elaine S. Costa1,2,*

1Clinical Medicine Postgraduate Program, College of Medicine, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil

2Cytometry Service, Institute of Pediatrics and Puericulture Martagão Gesteira (IPPMG), UFRJ, Rio de Janeiro, Brazil

3Departament of Medicine and Cytometry Service, Cancer Research Center (IBMCC, USAL-CSIC), Institute for Biomedical Research of Salamanca (IBSAL), University of Salamanca (USAL), Salamanca, Spain

4Cytogenetics Service, IPPMG-UFRJ and Polo Xerém-UFRJ, Rio de Janeiro, Brazil

5Cytogenetics Department, Bone Marrow Transplantation Unit and Oncology Post Graduation Program, National Cancer Institute (INCa), Rio de Janeiro, Brazil

6Department of Pediatric Hematology/Oncology, Medical University of Silesia, Zabrze, Poland

7Service of Pediatric Hematology, Federal Lagoa Hospital (HFL), Rio de Janeiro, Brazil

8Service of Pediatric Hematology, São José do Avaí Hospital (HSJA), Itaperuna, Rio de Janeiro, Brazil

9Service of Pediatric Hematology/Oncology, Servidores do Estado Federal Hospital (HSE), Rio de Janeiro, Brazil

10Service of Pediatric Hematology, Children’s Cancer Hospital of Barretos, Barretos, São Paulo, Brazil

*These authors contributed equally to this work

Correspondence to:

Elaine S. Costa, e-mail: elainesc.ufrj@gmail.com

Alberto Orfao, e-mail: orfao@usal.es

Keywords: B-cell precursor acute lymphoblastic leukemia, residual hematopoiesis, altered neutrophil immunophenotype, multiparameter flow cytometry, childhood

Received: December 22, 2015     Accepted: March 02, 2016     Published: March 25, 2016


An increasing number of evidences suggest a genetic predisposition in acute lymphoblastic leukemia (ALL) that might favor the occurrence of the driver genetic alterations. Such genetic background might also translate into phenotypic alterations of residual hematopoietic cells. Whether such phenotypic alterations are present in bone marrow (BM) cells from childhood B-cell precursor (BCP)-ALL remains to be investigated. Here we analyzed the immunophenotypic profile of BM and peripheral blood (PB) maturing/matured neutrophils from 118 children with BCP-ALL and their relationship with the features of the disease. Our results showed altered neutrophil phenotypes in most (77%) BCP-ALL cases. The most frequently altered marker was CD10 (53%), followed by CD33 (34%), CD13 (15%), CD15/CD65 (10%) and CD123 (7%). Of note, patients with altered neutrophil phenotypes had younger age (p = 0.03) and lower percentages of BM maturing neutrophils (p = 0.004) together with greater BM lymphocyte (p = 0.04), and mature B-cell (p = 0.03) counts. No significant association was found between an altered neutrophil phenotype and other disease features. These findings point out the potential existence of an altered residual hematopoiesis in most childhood BCP-ALL cases.

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