Oncotarget

Research Papers:

This article has been retracted. Retraction in: Oncotarget. 2018; 9:32551.

MicroRNA-371-5p targets SOX2 in gastric cancer

Yu-Ji Li _, Ming Dong, Fan-Min Kong, Jian-Ping Zhou, Dong Liang and Huan-Zhou Xue

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Oncotarget. 2016; 7:31993-32005. https://doi.org/10.18632/oncotarget.8289

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Abstract

Yu-Ji Li1, Ming Dong1, Fan-Min Kong1, Jian-Ping Zhou1, Dong Liang1, Huan-Zhou Xue1

1Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China

Correspondence to:

Yu-Ji Li, email: liyuji0930@yeah.net

Keywords: microRNA-371-5p, gastric cancer, SOX2, diological significance

Received: September 29, 2015     Accepted: March 04, 2016     Published: March 23, 2016

ABSTRACT

We evaluated miR-371-5p expression in gastric cancer (GC) tissues and its influence on the expression of downstream genes, especially SOX2. MiR-371-5p expression (measured using qRT-PCR) was upregulated in GC tissues and correlated positively with TNM staging and lymph node (LN) metastasis. MiR-371-5p expression was higher in human GC cell lines (AGS, MKN-28, BGC-823, MGC-803, SGC-7901 and MKN-45) than in human normal gastric epithelial (GES-1) cells (all P < 0.05). MGC-803 tumor cell growth (measured with an MTT assay), migration, and invasion (measured with Transwell chamber assays) were severely inhibited in cells transfected with a miR-371-5p inhibitor, whereas they were stimulated in cells transfected with SOX2 siRNA or miR-371-5p inhibitor + SOX2 siRNA. Expression of SOX2 mRNA and protein (assessed with qRT-PCR and Western blot) were greatly enhanced in the miR-371-5p inhibitor group. These results indicate that miR-371-5p expression is strongly upregulated in GC tissues and negatively correlated with SOX2 expression, while miR-371-5p expression is inversely related to proliferation, TNM stage, and LN metastasis of GC cells. Suppression of miR-371-5p may inhibit the growth and invasion of MGC-803 GC cells by upregulating SOX2 expression.


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