MicroRNA-371-5p targets SOX2 in gastric cancer
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Yu-Ji Li1, Ming Dong1, Fan-Min Kong1, Jian-Ping Zhou1, Dong Liang1, Huan-Zhou Xue1
1Department of General Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, P.R. China
Yu-Ji Li, email: [email protected]
Keywords: microRNA-371-5p, gastric cancer, SOX2, diological significance
Received: September 29, 2015 Accepted: March 04, 2016 Published: March 23, 2016
We evaluated miR-371-5p expression in gastric cancer (GC) tissues and its influence on the expression of downstream genes, especially SOX2. MiR-371-5p expression (measured using qRT-PCR) was upregulated in GC tissues and correlated positively with TNM staging and lymph node (LN) metastasis. MiR-371-5p expression was higher in human GC cell lines (AGS, MKN-28, BGC-823, MGC-803, SGC-7901 and MKN-45) than in human normal gastric epithelial (GES-1) cells (all P < 0.05). MGC-803 tumor cell growth (measured with an MTT assay), migration, and invasion (measured with Transwell chamber assays) were severely inhibited in cells transfected with a miR-371-5p inhibitor, whereas they were stimulated in cells transfected with SOX2 siRNA or miR-371-5p inhibitor + SOX2 siRNA. Expression of SOX2 mRNA and protein (assessed with qRT-PCR and Western blot) were greatly enhanced in the miR-371-5p inhibitor group. These results indicate that miR-371-5p expression is strongly upregulated in GC tissues and negatively correlated with SOX2 expression, while miR-371-5p expression is inversely related to proliferation, TNM stage, and LN metastasis of GC cells. Suppression of miR-371-5p may inhibit the growth and invasion of MGC-803 GC cells by upregulating SOX2 expression.
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