Oncotarget

Research Papers:

MiR-26a and miR-144 inhibit proliferation and metastasis of esophageal squamous cell cancer by inhibiting cyclooxygenase-2

Ying Shao _, Peng Li, Sheng-Tao Zhu, Ji-Ping Yue, Xiao-Jun Ji, Dan Ma, Li Wang, Yong-Jun Wang, Ye Zong, Yong-Dong Wu and Shu-Tian Zhang

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Oncotarget. 2016; 7:15173-15186. https://doi.org/10.18632/oncotarget.7908

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Abstract

Ying Shao1, Peng Li1, Sheng-Tao Zhu1, Ji-Ping Yue1, Xiao-Jun Ji2, Dan Ma1, Li Wang1, Yong-Jun Wang1, Ye Zong1, Yong-Dong Wu1, Shu-Tian Zhang1

1Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing, China

2Department of Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China

Correspondence to:

Shu-Tian Zhang, e-mail: [email protected]

Keywords: cyclooxygenase-2, esophageal squamous cell cancer, proliferation and metastasis, miR-26a, miR-144

Received: July 12, 2015     Accepted: January 29, 2016     Published: March 4, 2016

ABSTRACT

The altered expression of miRNAs is involved in carcinogenesis of esophageal squamous cell carcinoma (ESCC), but whether miRNAs regulate COX-2 expression in ESCC is not clear. To this end, the expression levels of miR-26a and miR-144 in ESCC clinical tissues and cell lines were investigated by qRT-PCR. COX-2 and PEG2 were quantified by western blot and ELISA. Decrease in miR-26a and miR-144 expression in ESCC was found by a comparison between 30 pairs of ESCC tumor and adjacent normal tissues as well as in 11 ESCC cell lines (P < 0.001). Co-transfection of miR-26a and miR-144 in ESCC cell lines more significantly suppressed cell proliferation, migration, and invasion than did either miR-26a or miR-144 alone (all P < 0.001), as shown by assays of CCK8, migration and invasion and flow cytometry. The inhibitory effect of these two miRNAs in vivo was also verified in nude mice xenograft models. COX-2 was confirmed as a target of miR-26a and miR-144. In conclusion, miR-26a and miR-144 expression is downregulated in ESCC. Co-expression of miR-26a and miR-144 in ESCC cells resulted in inhibition of proliferation and metastasis in vitro and in vivo, suggesting that targeting COX-2 may be the mechanism of these two miRNAs.


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