The clock gene PER1 suppresses expression of tumor-related genes in human oral squamous cell carcinoma
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Han-Xue Li1, Xiao-Juan Fu1, Kai Yang1, Dan Chen1, Hong Tang1, Qin Zhao1
1Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Kai Yang, e-mail: [email protected]
Keywords: oral cancer, tumor, circadian clock, PER1, tumor-related genes
Received: October 11, 2015 Accepted: February 20, 2016 Published: March 01, 2016
Abnormal expression of the clock gene PER1 is highly correlated with carcinogenesis and the development of malignant tumors. Here, we designed short hairpin RNAs (shRNAs) to effectively knock down PER1 in SCC15 human oral squamous cell carcinoma cells. shRNA-mediated PER1 knockdown promoted SCC15 cell growth, proliferation, apoptosis resistance, migration and invasion in vitro. PER1 knockdown also increased the cells’ expression of KI-67, MDM2, BCL-2, MMP2 and MMP9 mRNA, and decreased expression of C-MYC, p53, BAX and TIMP-2. In BALB/c nu/nu nude mice subcutaneously injected with SCC15 cells, PER1 knockdown in the cells enhanced tumor development, leading to increased tumor weights and volumes. These results suggest that PER1 is an important tumor suppressor gene and may be a useful molecular target for the treatment of cancer.
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