Research Papers:

Carcinoma-associated fucosylated antigens are markers of the epithelial state and can contribute to cell adhesion through CLEC17A (Prolectin)

Adrien Breiman _, María Dolores López Robles, Sophie de Carné Trécesson, Klara Echasserieau, Karine Bernardeau, Kurt Drickamer, Anne Imberty, Sophie Barillé-Nion, Frédéric Altare and Jacques Le Pendu

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Oncotarget. 2016; 7:14064-14082. https://doi.org/10.18632/oncotarget.7476

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Adrien Breiman1,2, María Dolores López Robles1, Sophie de Carné Trécesson1, Klara Echasserieau1,5, Karine Bernardeau1,5, Kurt Drickamer3, Anne Imberty4, Sophie Barillé-Nion1, Frédéric Altare1, Jacques Le Pendu1

1Inserm U892, CNRS UMR6299, University of Nantes, 44007 Nantes, France

2Nantes University Hospital, 44007 Nantes, France

3Department of Life Sciences, Imperial College London, London SW7, UK

4CERMAV-UPR 5301, CNRS, Université Grenoble Alpes, 38041 Grenoble, France

5Recombinant Protein Core Facility of The University of Nantes, 44007 Nantes, France

Correspondence to:

Adrien Breiman, e-mail: [email protected]

Jacques Le Pendu, e-mail: [email protected]

Keywords: fucose, lectins, epithelial to mesenchymal transition, lymph node metastasis

Received: July 31, 2015     Accepted: January 31, 2016     Published: February 18, 2016


Terminal fucosylated motifs of glycoproteins and glycolipid chains are often altered in cancer cells. We investigated the link between fucosylation changes and critical steps in cancer progression: epithelial-to-mesenchymal transition (EMT) and lymph node metastasis.

Using mammary cell lines, we demonstrate that during EMT, expression of some fucosylated antigens (e.g.: Lewis Y) is decreased as a result of repression of the fucosyltransferase genes FUT1 and FUT3. Moreover, we identify the fucose-binding bacterial lectin BC2L-C-Nt as a specific probe for the epithelial state.

Prolectin (CLEC17A), a human lectin found on lymph node B cells, shares ligand specificities with BC2L-C-Nt. It binds preferentially to epithelial rather than to mesenchymal cells, and microfluidic experiments showed that prolectin behaves as a cell adhesion molecule for epithelial cells. Comparison of paired primary tumors/lymph node metastases revealed an increase of prolectin staining in metastasis and high FUT1 and FUT3 mRNA expression was associated with poor prognosis. Our data suggest that tumor cells invading the lymph nodes and expressing fucosylated motifs associated with the epithelial state could use prolectin as a colonization factor.

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