Research Papers:

Identification of a long non-coding RNA NR_026689 associated with lung carcinogenesis induced by NNK

Jianjun Wu, Xun Li, Yiqin Xu, Ti Yang, Qiaoyuan Yang, Chengfeng Yang and Yiguo Jiang _

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Oncotarget. 2016; 7:14486-14498. https://doi.org/10.18632/oncotarget.7475

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Jianjun Wu1,*, Xun Li1,*, Yiqin Xu1, Ti Yang1, Qiaoyuan Yang1, Chengfeng Yang2, Yiguo Jiang1

1State Key Laboratory of Respiratory Disease, Institute for Chemical Carcinogenesis, Guangzhou Medical University, Guangzhou 511436, People’s Republic of China

2Department of Physiology and Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA

*These authors have contributed equally to this work

Correspondence to:

Yiguo Jiang, e-mail: [email protected]

Keywords: lncRNA, NR_026689, lung carcinogenesis, NNK

Received: September 05, 2015     Accepted: January 29, 2016     Published: February 18, 2016


Long non-coding RNAs (lncRNA) are thought to be important epigenetic regulators involved in the development of a variety of cancers. Alterations in lncRNA expression are associated with exposure to chemical carcinogens. However, it is still unclear whether lncRNA expression during lung carcinogenesis is induced by chemical carcinogens. In this study, using NNK-induced rat lung cancer model established by our previous study, we determined the lncRNA expression profiles, and an alteration in lncRNA expression was observed in lung cancer tissues and blood in the NNK treatment group. Using quantitative reverse-transcription PCR (qRT-PCR), five differentially expressed lncRNAs were further detected and validated. We identified a novel lncRNA, NR_026689, which showed increased expression in lung cancer tissues induced by NNK and the alteration of lncRNA NR_026689 was specifically observed in lung tissue. The level of NR_026689 was determined and significantly increased in rat whole blood at the 10th and 20th week after NNK treatment to evaluate it as a potential early marker for lung cancer. Together, these findings suggest that lncRNA NR_026689 may be a potential early biomarker for lung cancer and is associated with lung carcinogenesis induced by NNK.

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