Research Papers:
EIF2S3Y suppresses the pluripotency state and promotes the proliferation of mouse embryonic stem cells
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Na Li1, Hailong Mu1, Liming Zheng1, Bo Li1, Chongyang Wu1, Bowen Niu1, Qiaoyan Shen1, Xin He1, Jinlian Hua1
1College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A & F University, Yangling, Shaanxi, China
Correspondence to:
Jinlian Hua, e-mail: [email protected]
Keywords: EIF2S3Y, embryonic stem cells, pluripotency, proliferation, mouse
Received: October 08, 2015 Accepted: January 23, 2016 Published: February 04, 2016
ABSTRACT
Eukaryotic translation initiation factor 2, subunit 3, and structural gene Y-linked (EIF2S3Y) is essential for spermatogenesis in mouse models. However, its effect on embryonic stem (ES) cells remains unknown. In our observation, differentiated ES cells showed higher levels of EIF2S3Y. To further elucidate its role in ES cells, we utilized ES-derived EIF2S3Y-overexpressing cells and found that EIF2S3Y down-regulated the pluripotency state of ES cells, which might be explained by decreased histone methylation levels because of reduced levels of ten-eleven translocation 1 (TET1). Moreover, EIF2S3Y-overexpressing cells showed an enhanced proliferation rate, which might be due to increased Cyclin A and Cyclin E levels. This study highlighted novel roles of EIF2S3Y in the pluripotency maintenance and proliferation control of ES cells, which would provide an efficient model to study germ cell generation as well as cancer development using ES cells, thus providing valuable target for clinical applications of ES cells.