Oncotarget

Research Papers:

Role of CD44high/CD133high HCT-116 cells in the tumorigenesis of colon cancer

Jin-Yong Zhou, Min Chen, Long Ma, Xiaoxiao Wang, Yu-Gen Chen and Shen-Lin Liu _

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Oncotarget. 2016; 7:7657-7666. https://doi.org/10.18632/oncotarget.7084

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Abstract

Jin-Yong Zhou1,*, Min Chen2,*, Long Ma3,*, Xiaoxiao Wang4, Yu-Gen Chen5,*, Shen-Lin Liu6

1Central Laboratory, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

2Department of Internal Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

3Department of Urology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

4Department of Medical Science Research, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

5Department of Colorectal Surgery, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

6Department of Oncology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

*These authors contributed equally to this work

Correspondence to:

Shen-Lin Liu, e-mail: jsszyylsl@163.com

Keywords: CD133, CD44, cancer stem cells (CSCs), colorectal cancer, biomarkers

Received: September 24, 2015     Accepted: January 13, 2016     Published: January 30, 2016

ABSTRACT

This study aimed to explore cell surface biomarkers related to cancer stem cells (CSCs) and their role in the tumorigenesis of colon cancer. Various colon cancer cell lines were screened for CD133 and CD44 expression. CD44high/CD133high and CD44low/CD133low cells were separately isolated by Fluorescence-Activated Cell Sorting (FACS). The cell proliferation, colony formation, cell cycle characteristics, and tumorigenic properties in CD44high/CD133high and CD44low/CD133low cells were investigated through in vitro experiments and in vivo tumor xenograft models. The expression profiles of stem cell-related genes were examined by RT-PCR. With HCT-116 cells, flow cytometry analysis revealed that CD44high/CD133high cells had higher proliferation potency than CD44low/CD133low cells. Compared to CD44low/CD133low cells, CD44high/CD133high cells had more stem cell-related genes, and displayed increased tumorigenic ability. In summary, CD44high/CD133high cells isolated from HCT-116 cells harbor CSC properties that may be related to the tumor growth of colon cancer. These results suggest that CD44 and CD133 could be strong markers of colorectal cancer stem cells.


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