Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma
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Bingchang Zhang1, Fen Wang1, Lianzhi Dai1, Heguo Cai2, Yanyan Zhan1, Song Gang1, Tianhui Hu1, Chun Xia2, Bing Zhang1
1School of Medicine, Xiamen University, Fujian, 361102, China
2Zhongshan Hospital, Xiamen University, Fujian, 361004, China
Chun Xia, e-mail: [email protected]
Bing Zhang, e-mail: [email protected]
Keywords: PLCγ1, ShRNA, tumor growth, tumor metastasis, human gastric adenocarcinoma cells
Received: September 09, 2015 Accepted: January 01, 2016 Published: January 22, 2016
Targeted molecular therapy has gradually been a potential solution in cancer therapy. Other authors’ and our previous studies have demonstrated that phosphoinositide-specific phospholipase γ (PLCγ) is involved in regulating tumor growth and metastasis. However, the molecular mechanism underlying PLCγ-dependent tumor growth and metastasis of gastric adenocarcinoma and whether PLCγ may be a potential target for tumor therapy in human gastric adenocarcinoma are not yet well determined. Here, we investigated the role of PLCγ inhibition in tumor growth and metastasis of human gastric adenocarcinoma using BGC-823 cell line and a nude mouse tumor xenograft model. The results manifested that the depletion of PLCγ1 by the transduction with lentivirus-mediated PLCγ1 gene short-hairpin RNA (shRNA) vector led to the decrease of tumor growth and metastasis of human gastric adenocarcinoma in vitro and in vivo. Furthermore, the Akt/Bad, Akt/S6, and ERK/Bad signal axes were involved in PLCγ1-mediated tumor growth and metastasis of human gastric adenocarcinoma. Therefore, the abrogation of PLCγ1 signaling by shRNA could efficaciously suppress human gastric adenocarcinoma tumor growth and metastasis, with important implication for validating PLCγ1 as a potential target for human gastric adenocarcinoma.
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