Oncotarget

Research Papers:

Cell cycle-dependent resolution of DNA double-strand breaks

Susanna Ambrosio, Giacomo Di Palo, Giuliana Napolitano, Stefano Amente, Gaetano Ivan Dellino, Mario Faretta, Pier Giuseppe Pelicci, Luigi Lania _ and Barbara Majello

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2016; 7:4949-4960. https://doi.org/10.18632/oncotarget.6644

Metrics: PDF 1271 views  |   HTML 1575 views  |   ?  


Abstract

Susanna Ambrosio1, Giacomo Di Palo2, Giuliana Napolitano1, Stefano Amente2, Gaetano Ivan Dellino3,4, Mario Faretta3, Pier Giuseppe Pelicci3,4, Luigi Lania2, Barbara Majello1

1Department of Biology, University of Naples ‘Federico II’, Naples, Italy

2Department of Molecular Medicine and Medical Biotechnologies, University of Naples ‘Federico II’, Naples, Italy

3Department of Experimental Oncology, European Institute of Oncology, Milan, Italy

4Department of Oncology and Haemato-oncology, University of Milan, Italy

Correspondence to:

Luigi Lania, e-mail: lania@unina.it

Barbara Majello, e-mail: majello@unina.it

Keywords: cell-cycle, DSB repair, site-specific DSBs, AsiSI restriction enzyme

Received: November 05, 2015     Accepted: November 27, 2015     Published: December 17, 2015

ABSTRACT

DNA double strand breaks (DSBs) elicit prompt activation of DNA damage response (DDR), which arrests cell-cycle either in G1/S or G2/M in order to avoid entering S and M phase with damaged DNAs. Since mammalian tissues contain both proliferating and quiescent cells, there might be fundamental difference in DDR between proliferating and quiescent cells (or G0-arrested). To investigate these differences, we studied recruitment of DSB repair factors and resolution of DNA lesions induced at site-specific DSBs in asynchronously proliferating, G0-, or G1-arrested cells. Strikingly, DSBs occurring in G0 quiescent cells are not repaired and maintain a sustained activation of the p53-pathway. Conversely, re-entry into cell cycle of damaged G0-arrested cells, occurs with a delayed clearance of DNA repair factors initially recruited to DSBs, indicating an inefficient repair when compared to DSBs induced in asynchronously proliferating or G1-synchronized cells. Moreover, we found that initial recognition of DSBs and assembly of DSB factors is largely similar in asynchronously proliferating, G0-, or G1-synchronized cells. Our study thereby demonstrates that repair and resolution of DSBs is strongly dependent on the cell-cycle state.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 6644