Regulatory T cells may participate in Helicobacter pylori persistence in gastric MALT lymphoma: lessons from an animal model
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Amandine Marine Laur1,2,*, Pauline Floch1,2,*, Lucie Chambonnier1,2, Lucie Benejat1, Victoria Korolik3, Alban Giese4, Pierre Dubus4, Francis Mégraud1,2, Antonio Bandeira5, Philippe Lehours1,2
1University Bordeaux, Bacteriology Laboratory, Bordeaux, France
2INSERM U853, Bordeaux, France
3Institute for Glycomics, Griffith University, Nathan QLD, Australia
4University Bordeaux, EA 2406, Bordeaux, France
5Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, Paris, France
*These authors have contributed equally to this work
Philippe Lehours, e-mail: [email protected]
Keywords: MALT lymphoma, regulatory T cell, animal model, Helicobacter pylori
Received: August 04, 2015 Accepted: November 16, 2015 Published: December 07, 2015
It has been postulated that the emergence of autoimmune gastritis in neonatal thymectomised (d3Tx) BALB/c mice may be a consequence of post-surgery deficit in Tregs. In this study, previously obtained samples from d3Tx mice were used in order to determine whether thymectomy creates a deficit in this T cell subset thereby allowing the emergence of autoimmune phenomena as a prerequisite for GML. The splenic Treg reserve and the local recruitment of these cells in the gastric mucosa were investigated using complementary molecular and immunohistochemistry approaches. Higher Foxp3/CD3 ratios were found in the spleen of non-infected d3Tx mice compared to non-thymectomised (NTx) controls. These results indicate a relative enrichment of Tregs following thymectomy in adult mice. The absence of Treg depletion in d3Tx mice is in line with the absence of auto-immune gastritis in non-infected d3Tx mice. Higher levels of T cell and Treg infiltration were also found in the stomach of GML-developing d3Tx mice versus NTx mice. Surprisingly, inflammatory scores inversely correlated with the bacterial inoculum. The presence of a small Treg containing compartment among gastric biopsies of GML developing d3Tx mice may play a role in perseverance of a minimal bacterial numbers thereby maintaining an antigen-dependent stimulation and proliferation.
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