Whole-genome duplication increases tumor cell sensitivity to MPS1 inhibition
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Mohamed Jemaà1, Gwenola Manic2, Gwendaline Lledo1, Delphine Lissa3,4,5,6, Christelle Reynes7, Nathalie Morin1, Frédéric Chibon8,9, Antonella Sistigu2, Maria Castedo3,4,5,6, Ilio Vitale2,10,*, Guido Kroemer4,5,11,12,13,*, Ariane Abrieu1,*
1CRBM, CNRS UMR5237, Université de Montpellier, Montpellier, France
2Regina Elena National Cancer Institute, Rome, Italy
3Université Paris-Sud/Paris XI, Le Kremlin-Bicêtre, France
4INSERM, UMRS1138, Paris, France
5Equipe 11 Labelisée par la Ligue Nationale Contre le Cancer, Centre de Recherche des Cordeliers, Paris, France
6Gustave Roussy Cancer Campus, Villejuif, France
7EA 2415, Laboratoire de Biostatistique, d’Epidémiologie et de Recherche Clinique, Université de Montpellier, Montpellier, France
8Department of Biopathology, Institut Bergonié, Comprehensive Cancer Centre, Bordeaux, France
9INSERM U916, Bordeaux, France
10Department of Biology, University of Rome “Tor Vergata”, Rome, Italy
11Université Pierre et Marie Curie/Paris VI, Paris, France
12Pôle de Biologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France
13Metabolomics and Cell Biology Platforms, Gustave Roussy Cancer Campus, Villejuif, France
*I.V., G.K., and A.A. share co-senior authorship
Ilio Vitale, e-mail: [email protected]
Guido Kroemer, e-mail: [email protected]
Ariane Abrieu, e-mail: [email protected]
Keywords: AZ 3146, mitotic spindle, polyploidy, regulated cell death, reversine
Received: June 25, 2015 Accepted: November 18, 2015 Published: November 30, 2015
Several lines of evidence indicate that whole-genome duplication resulting in tetraploidy facilitates carcinogenesis by providing an intermediate and metastable state more prone to generate oncogenic aneuploidy. Here, we report a novel strategy to preferentially kill tetraploid cells based on the abrogation of the spindle assembly checkpoint (SAC) via the targeting of TTK protein kinase (better known as monopolar spindle 1, MPS1). The pharmacological inhibition as well as the knockdown of MPS1 kills more efficiently tetraploid cells than their diploid counterparts. By using time-lapse videomicroscopy, we show that tetraploid cells do not survive the aborted mitosis due to SAC abrogation upon MPS1 depletion. On the contrary diploid cells are able to survive up to at least two more cell cycles upon the same treatment. This effect might reflect the enhanced difficulty of cells with whole-genome doubling to tolerate a further increase in ploidy and/or an elevated level of chromosome instability in the absence of SAC functions. We further show that MPS1-inhibited tetraploid cells promote mitotic catastrophe executed by the intrinsic pathway of apoptosis, as indicated by the loss of mitochondrial potential, the release of the pro-apoptotic cytochrome c from mitochondria, and the activation of caspases. Altogether, our results suggest that MPS1 inhibition could be used as a therapeutic strategy for targeting tetraploid cancer cells.
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