Oncotarget

Research Papers: Pathology:

In vivo regeneration of renal vessels post whole decellularized kidneys transplantation

JianSe Zhang, ZhiBin Wang, KeZhi Lin, YaLing Yu, LiNa Zhao, TingGang Chu, LiZhi Wu, Ali Alkhawaji, MiaoZhong Li, YingKuan Shao, Ting Li, XinFa Lou, ShiXin Chen, MaoLin Tang and Jin Mei _

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Oncotarget. 2015; 6:40433-40442. https://doi.org/10.18632/oncotarget.6321

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Abstract

JianSe Zhang1,2,*, ZhiBin Wang1,*, KeZhi Lin1, YaLing Yu1,2, LiNa Zhao2, TingGang Chu3, LiZhi Wu4, Ali Alkhawaji1,5, MiaoZhong Li1, YingKuan Shao1, Ting Li1,2, XinFa Lou2, ShiXin Chen2, MaoLin Tang1,2 and Jin Mei1,2,6

1 Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China

2 Anatomy Department, Wenzhou Medical University, Wenzhou, China

3 Department of Orthopedics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China

4 Department of Hand and Foot Surgery, Luqiao Hospital of Enze Medical Center, Taizhou, China

5 Department of Anatomy, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia

6 Institute of Neuroscience, Wenzhou Medical University, Wenzhou, China

* These authors have contributed equally to this work

Correspondence to:

Jin Mei, email:

MaoLin Tang, email:

Keywords: renal vessels, scaffold, microsurgery, orthotopic transplantation, renal regeneration, Pathology Section

Received: May 15, 2015 Accepted: October 21, 2015 Published: November 13, 2015

Abstract

Nearly 50 million patients in China live with end-stage renal disease (ESRD), and only about 4000 patients may receive kidney transplantation. The purpose of this study was to investigate regeneration of renal vessels post whole decellularized kidneys transplantation in vivo. We decellularized kidneys of donor rats by perfusing a detergent through the abdominal aorta, yielding feasible extracellular matrix, confirmed for acellularity before transplantation. Based on the concept of using the body as a bioreactor, we orthotopically transplanted the kidney and ureter scaffolds in recipient rats, and found the regeneration of vessels including artery and vein in the renal sinus following a spontaneous recanalization. Although the findings only represent an initial step toward the ultimate goal of the generation of fully functional kidneys in vivo, these findings suggest that the body itself, as the bioreactor, is a viable strategy for kidney regeneration.


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